Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng
| Autor: | Jae Youl Cho, Seong-Gu Jeong, Junseong Park, Sunggyu Kim, Yo Han Hong, Sulgi Yoo, Gibaeg Nam, Sang Yun Han, Deok Jeong, Dong-Hyun Kim, Eunji Kim, Keejung Yoon |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
photoaging Photoaging Fraction (chemistry) Biology Biochemistry Genetics and Molecular Biology (miscellaneous) UVB irradiation 03 medical and health sciences Ginseng 0302 clinical medicine lcsh:Botany medicine Compound K Uvb irradiation BIOGF1K integumentary system Traditional medicine anti-aging Panax ginseng medicine.disease lcsh:QK1-989 030104 developmental biology Complementary and alternative medicine Biochemistry 030220 oncology & carcinogenesis Biotechnology |
| Zdroj: | Journal of Ginseng Research, Vol 42, Iss 1, Pp 81-89 (2018) |
| ISSN: | 1226-8453 |
| DOI: | 10.1016/j.jgr.2017.01.002 |
| Popis: | Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events. Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry. We also evaluated the effects of BIOGF1K on the activator protein-1 signaling pathway, which plays an important role in photoaging, by immunoblot analysis and luciferase reporter gene assays. Results: Treatment of UVB-irradiated NIH3T3 fibroblasts with BIOGF1K prevented UVB-induced cell death, inhibited apoptosis, suppressed morphological changes, reduced melanin secretion, restored the levels of type I procollagen and sirtuin 1, and prevented mRNA upregulation of MMP-1, MMP-2, and cyclo-oxygenase-2; these effects all occurred in a dose-dependent manner. In addition, BIOGF1K markedly reduced activator-protein-1-mediated luciferase activity and decreased the activity of mitogen-activated protein kinases (extracellular response kinase, p38, and C-Jun N-terminal kinase). Conclusion: Our results strongly suggest that BIOGF1K has anti-photoaging activity and that BIOGF1K could be used in anti-aging cosmeceutical preparations. |
| Databáze: | OpenAIRE |
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