Evaluation of p16/Ki-67 dual staining in the detection of cervical precancer and cancer in China
| Autor: | Xibin Sun, Yu-Ling Liu, Xiaoqin Cao, Dong-Mei Zhao, Wen Chen, Shaokai Zhang, Qiong Chen, Zhen Guo, Pei-Pei Guo, Zeni Wu, Man-Man Jia, Shuzheng Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Cancer Research medicine.medical_specialty China Epidemiology Biopsy Cervical precancer Uterine Cervical Neoplasms Gastroenterology Sensitivity and Specificity 03 medical and health sciences Young Adult 0302 clinical medicine Pregnancy Internal medicine Cytology Medicine Humans 030212 general & internal medicine Cyclin-Dependent Kinase Inhibitor p16 Early Detection of Cancer Aged Colposcopy biology medicine.diagnostic_test Staining and Labeling business.industry Papillomavirus Infections Cancer Gold standard (test) Middle Aged medicine.disease Uterine Cervical Dysplasia Dual staining female genital diseases and pregnancy complications Staining Ki-67 Antigen Oncology 030220 oncology & carcinogenesis Ki-67 biology.protein Female business |
| Zdroj: | Cancer epidemiology. 59 |
| ISSN: | 1877-783X |
| Popis: | Background This study aimed to evaluate the clinical performance of p16/Ki-67 dual staining in the detection of cervical intraepithelial neoplasia grade 2 or 3 or worse (CIN2+/CIN3+) in Chinese women. Methods Cervical exfoliated cells were collected from 537 eligible women and were used for liquid-based cytology (LBC), p16/Ki-67 dual staining, and human papillomavirus (HPV) DNA testing. All women received colposcopy with biopsies taken at abnormal sites. Histopathological diagnoses were used as the gold standard. Results p16/Ki-67 staining had a positivity rate of 43.58% overall; the rate increased significantly with histological severity (p0.001). The sensitivities of p16/ki-67 for detecting CIN2+ and CIN3+ were 88.10% and 91.30%, respectively. Compared with high-risk HPV (HR-HPV), sensitivity of p16/Ki-67 was lower for detecting CIN2+ (88.10% versus 95.71%), but similar for detecting CIN3+ (91.30% versus 96.27%). Specificities of p16/Ki-67 were 85.02% for detecting CIN2+ and 76.86% for detecting CIN3+, values similar to those for LBC (84.71% for CIN2+, 80.05% for CIN3+) but higher than those for HR-HPV (62.77% for CIN2+, 71.25% for CIN3+). All the tests performed better in women30 years. With respect to the performance of triage for women with ASC-US, sensitivities of p16/Ki-67 were 86.36% for detecting CIN2+ and 83.33% for detecting CIN3+, values similar to those of HR-HPV. However, specificities of p16/Ki-67 were both higher than those of HR-HPV (85.96% versus 67.54% for CIN2+, 79.84% versus 62.90% for CIN3+). Conclusion P16/Ki-67 dual staining could probably provide an optional method for China's national cervical cancer screening, and could also be considered as an efficient method of triage for managing women with ASC-US. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect