HPLC-DAD Analysis, Antileishmanial, Antiproliferative, and Antibacterial Activities of Lacistema pubescens: An Amazonian Medicinal Plant
| Autor: | Elaine M. Souza-Fagundes, Elaine Soares Coimbra, Antônia Ribeiro, Josiane Mello da Silva, Elita Scio, Luciana M.R. Antinarelli, Nícolas de Castro Campos Pinto |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Article Subject
Cell Survival lcsh:Medicine Antineoplastic Agents HL-60 Cells Biology Bacterial Physiological Phenomena General Biochemistry Genetics and Molecular Biology Lethal Dose 50 Phytol chemistry.chemical_compound Jurkat Cells Botany Humans Amastigote Cytotoxicity IC50 Chromatography High Pressure Liquid Cell Proliferation Leishmania Plants Medicinal General Immunology and Microbiology Traditional medicine Dose-Response Relationship Drug Plant Extracts lcsh:R General Medicine Neoplasms Experimental Antimicrobial biology.organism_classification Anti-Bacterial Agents Tracheophyta chemistry Antibacterial activity Bacteria Brazil Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Species of the genusLacistemaare traditionally used by Brazilian and Peruvian indigenous communities. The present study investigated thein vitroantileishmanial activity against severalLeishmaniaspecies, cytotoxicity in murine peritoneal macrophages, antiproliferative activity against HL60 and Jurkat cells, and antibacterial activities against seven bacteria strains of the aerial parts of the methanolic crude extract and fractions ofLacistema pubescens. In addition, their chemical profile was also evaluated. Hexane fraction showed the most significant IC50values against all promastigotes ofLeishmaniaspecies tested, except forL. chagasi(IC50= 4.2 µg/mL forL. majorand IC50= 3.5 µg/mL forL. amazonensis). This fraction also exhibited a strong activity against amastigotes ofL. amazonensis(IC50= 6.9 µg/mL). The antiproliferative activity was also observed for methanolic extract and hexane fraction with IC50= 47.2 µg/mL and IC50= 39.7 µg/mL for HL60, respectively. Regarding the antimicrobial activity, the overall antibacterial activity was not very significative. Phytol and sitosterol were identified in the methanolic extract. Additionally, previous studies also revealed the presence of those compounds in the hexane fraction. Among other compounds, phytol and sitosterol were probably involved in the antileishmanial and cytotoxicity activities observed in this study. |
| Databáze: | OpenAIRE |
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