Combining transcriptional profiling and genetic linkage analysis to uncover gene networks operating in hematopoietic stem cells and their progeny
| Autor: | Brad Dykstra, Marcel Otten, Alice Gerrits, Leonid Bystrykh, Gerald de Haan |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
EXPRESSION
Transcriptional profiling Transcription Genetic Genetic Linkage Immunology Cell fate decisions Gene regulatory network TRAIT ANALYSIS Genomics Review Cell fate determination Biology MOUSE Stem cell marker 03 medical and health sciences 0302 clinical medicine HUMAN SOMATIC-CELLS HUMAN FIBROBLASTS Genetics Humans Cell Lineage Gene Regulatory Networks Gene LIFE-SPAN Oligonucleotide Array Sequence Analysis 030304 developmental biology Regulation of gene expression 0303 health sciences Genome Human Gene Expression Profiling COLONY-STIMULATING FACTOR Hematopoietic Stem Cells NERVOUS-SYSTEM Gene expression profiling MICE Gene Expression Regulation Genetic linkage analysis 030220 oncology & carcinogenesis Genetical genomics DEFINED FACTORS Stem cell |
| Zdroj: | Immunogenetics |
| ISSN: | 1432-1211 0093-7711 |
| Popis: | Stem cells are unique in that they possess both the capacity to self-renew and thereby maintain their original pool as well as the capacity to differentiate into mature cells. In the past number of years, transcriptional profiling of enriched stem cell populations has been extensively performed in an attempt to identify a universal stem cell gene expression signature. While stem-cell-specific transcripts were identified in each case, this approach has thus far been insufficient to identify a universal group of core "stemness" genes ultimately responsible for self-renewal and multipotency. Similarly, in the hematopoietic system, comparisons of transcriptional profiles between different hematopoietic cell stages have had limited success in revealing core genes ultimately responsible for the initiation of differentiation and lineage specification. Here, we propose that the combined use of transcriptional profiling and genetic linkage analysis, an approach called "genetical genomics", can be a valuable tool to assist in the identification of genes and gene networks that specify "stemness" and cell fate decisions. We review past studies of hematopoietic cells that utilized transcriptional profiling and/or genetic linkage analysis, and discuss several potential future applications of genetical genomics. |
| Databáze: | OpenAIRE |
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