Synthesis and quality assurance of [11C]alpha-aminoisobutyric acid (AIB), a potential radiotracer for imaging and amino acid transport studies in normal and malignant tissues
| Autor: | Pat Zanzonico, Boby M. Sundoro-Wu, Rodney E. Bigler, Jon K. Jacobsen, J. Robert Dahl, Bernard Schmall, Richard K. Lee, Peter S. Conti |
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| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
Ammonium carbonate Chromatography Quality Assurance Health Care Aminobutyrates Cyanide High-performance liquid chromatography Amino acid Endotoxins chemistry.chemical_compound chemistry Biochemistry In vivo Isotope Labeling Neoplasms Mole Acetone Humans Radiology Nuclear Medicine and imaging Carbon Radioisotopes Potassium Cyanide Radionuclide Imaging Chromatography High Pressure Liquid Limulus Test Amino acid synthesis |
| Zdroj: | International Journal of Nuclear Medicine and Biology. 11:209-214 |
| ISSN: | 0047-0740 |
| DOI: | 10.1016/0047-0740(84)90001-9 |
| Popis: | Carbon-I1 labeled alpha-aminoisobutyric acid (AIB), a synthetic amino acid, was prepared by the modified Bucherer-Strecker amino acid synthesis from acetone, ammonium carbonate and [ 11 C]KCN in the presence of carrier KCN. This method results in the labeling of AIB in the carboxyl group. The label is stable in this position because AIB is not a metabolized after cellular uptake. AIB is rapidly accumulated in viable cells including malignant cells. Since it is a non-metabolized amino acid, AIB offers the possibility of studying amino acid transport in vivo without interference by radiolabeled metabolic products. Radiochemical yields of [ 11 C]AIB of 35–60% have been obtained in 70–80 min with radiopurities > 99% Carrier added syntheses gave 15–25 mCi of [ 11 C]AIB with specific activities of 0.3 Ci/mmol. Our quality control program which insures that [ 11 C]AIB is suitable for imaging studies in patients with cancer includes HPLC analyses of product identity and purity, apyrogenecity and isotonicity assays, and a sensitive test for cyanide. |
| Databáze: | OpenAIRE |
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