Development of Suitable ISE Measurement Procedures for SI-Traceable Chemical Activity Determination
| Autor: | Hanspeter Andres, Samuel Wunderli, Daniel Berdat |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ion-selective electrode
Potentiometric titration Analytical chemistry Amount of substance 010402 general chemistry 01 natural sciences Clinical chemistry Ion selective electrode Electrolyte Intensive care Calibration Process engineering QD1-999 Reliability (statistics) business.industry Chemistry Scale (chemistry) 010401 analytical chemistry General Medicine General Chemistry 0104 chemical sciences Certified reference materials Pitzer activity Potentiometry business |
| Zdroj: | CHIMIA, Vol 63, Iss 10 (2009) |
| ISSN: | 2673-2424 0009-4293 |
| DOI: | 10.2533/chimia.2009.670 |
| Popis: | In clinical chemistry and medical diagnostics, the reliability of the results obtained by numerous testing protocols has a major impact on the critical decision-making processes. Considerable efforts are made by international institutes to promote and give guidance on measurement comparisons and metrological traceability using appropriate certified reference materials. The concentration values or amount of substance contents are usually assigned to chemical analytical results. In contrast, the most relevant quantity is the activity of ions in clinical chemistry. Activity corresponds to the available biologically active part of these components in a mixture. Many measurement methods and instruments currently in use do not differentiate between the total substance concentration and its biologically active component. In physiological solutions, such as blood plasma, the chemical activity makes a clear difference between ion or substrate concentration. Ion selective electrodes (ISE) are widely used to directly measure the activity of ions. Measurement by means of ion-selective electrodes is a standard method in point of care testing units (POCT) for continuous monitoring for e.g. haemodialysis, cardiac surgery and in intensive care units. A metrological approach to ISE potentiometric measurements is proposed, to determine the activity with their uncertainty. A prerequisite is to develop an activity calibration scale for complex electrolyte solutions with known and traceable ion activities. |
| Databáze: | OpenAIRE |
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