Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation
| Autor: | Anja Bauermeister, Petra Rettberg, Ralf Moeller, Esma Bentchikou |
|---|---|
| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
Environmental UV radiation
Mutant medicine.disease_cause Biochemistry MESH: Dose-Response Relationship Drug chemistry.chemical_compound Deinococcus radiodurans 254 nm UV-C MESH: Bacterial Proteins MESH: DNA Repair 0303 health sciences Mutation MESH: Oxidative Stress biology General Medicine MESH: Deinococcus Anti-Bacterial Agents [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Rec A Recombinases Deinococcus MESH: Mutation DNA damage DNA repair Ultraviolet Rays Microbiology 03 medical and health sciences Bacterial Proteins MESH: Anti-Bacterial Agents MESH: Dose-Response Relationship Radiation MESH: Drug Resistance Bacterial Drug Resistance Bacterial Genetics medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Irradiation Molecular Biology 030304 developmental biology MESH: DNA Damage Dose-Response Relationship Drug 030306 microbiology Dose-Response Relationship Radiation biology.organism_classification Oxidative Stress Rec A Recombinases chemistry Biophysics MESH: Ultraviolet Rays Oxidative stress DNA DNA Damage |
| Zdroj: | Archiv für Mikrobiologie Archiv für Mikrobiologie, 2009, 191 (12), pp.913-8. ⟨10.1007/s00203-009-0522-7⟩ |
| DOI: | 10.1007/s00203-009-0522-7 |
| Popis: | International audience; To study the role of different DNA repair genes in the resistance of Deinococcus radiodurans to mono- and polychromatic UV radiation, wild-type strain and knockout mutants in RecA, PprA, and IrrE of D. radiodurans were irradiated with UV-C (254 nm), UV-(A + B) (280-400 nm) and UV-A (315-400 nm) radiation, and survival was monitored. The strain deficient in recA was highly sensitive to UV-C radiation compared to the wild-type, but showed no loss of resistance against irradiation with UV-(A + B) and UV-A, while pprA and irrE-deficient strains exhibited elevated sensitivity to UV-A and UV-(A + B) radiation. These results suggest that the repair of DNA double-strand breaks is essential after treatment with highly energetic UV-C radiation, whereas protection from oxidative stress may play a greater role in resistance to environmentally relevant UV radiation. |
| Databáze: | OpenAIRE |
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