Global gene expression profiling of the asymptomatic bacteriuria Escherichia coli strain 83972 in the human urinary tract
| Autor: | Viktoria Roos, Per Klemm |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Bacteriuria Virulence Factors Urinary system Iron Immunology medicine.disease_cause Nitric Oxide Microbiology Nitrate Reductase Sugar acids Gene expression medicine Escherichia coli Humans Amino Acids Urinary Tract Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Regulation of gene expression biology Gene Expression Profiling Gene Expression Regulation Bacterial biology.organism_classification medicine.disease Enterobacteriaceae Molecular Pathogenesis Gene expression profiling Infectious Diseases chemistry Fimbriae Bacterial Carbohydrate Metabolism Parasitology Female |
| Zdroj: | Infection and immunity. 74(6) |
| ISSN: | 0019-9567 |
| Popis: | Urinary tract infections (UTIs) are an important health problem worldwide, with many million cases each year. Escherichia coli is the most common organism causing UTIs in humans. The asymptomatic bacteriuria E. coli strain 83972 is an excellent colonizer of the human urinary tract, where it causes long-term bladder colonization. The strain has been used for prophylactic purposes in patients prone to more severe and recurrent UTIs. For this study, we used DNA microarrays to monitor the expression profile of strain 83972 in the human urinary tract. Significant differences in expression levels were seen between the in vivo expression profiles of strain 83972 in three patients and the corresponding in vitro expression profiles in lab medium and human urine. The data revealed an in vivo lifestyle of microaerobic growth with respiration of nitrate coupled to degradation of sugar acids and amino acids, with no signs of attachment to host tissues. Interestingly, genes involved in NO protection and metabolism showed significant up-regulation in the patients. This is one of the first studies to address bacterial whole-genome expression in humans and the first study to investigate global gene expression of an E. coli strain in the human urinary tract. |
| Databáze: | OpenAIRE |
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