Molecular variants of human Ia-like antigens
| Autor: | R. Kositsky, A. K. Sullivan, R. L. Ikeman, K. Bartok, L. M. Jerry |
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| Rok vydání: | 1978 |
| Předmět: |
HLA-D Antigens
Gel electrophoresis chemistry.chemical_classification B-Lymphocytes Isoantigens Multidisciplinary Isoelectric focusing Lymphocyte Genes MHC Class II Biology Molecular biology Cell Line Leukemia Lymphoid Molecular Weight medicine.anatomical_structure Immune system Membrane protein chemistry Antigen medicine Humans Electrophoresis Polyacrylamide Gel Isoelectric Focusing Glycoprotein |
| Zdroj: | Nature. 272(5650) |
| ISSN: | 0028-0836 |
| Popis: | THE Ia antigens, a group of plasma membrane proteins predominately expressed on B lymphocytes and thought to be products of the ‘immune response (Ir) region’ of the major histocompatibility complex1–4, are believed to play a part in the mechanism of T–B cell cooperation1,5, stimulation in mixed lymphocyte culture1, graft-versus-host reactions1 and T-cell suppression6. They have been demonstrated on the surface of a variety of cells which take part in intercellular interactions, such as B lymphocytes, macrophages, suppressor T cells, sperm7 and Langerhans cells of the skin8. In several species these glycoproteins exist in a complex containing 33,000 and 27,000 molecular weight (MW) moieties9,10. Although Snary et al.11 have shown that the human alloantigens may reside predominantly on the 33,000 MW chain, alloantisera are able to precipitate both components. Attempts to show molecular variants within either chain by immune precipitation with alloantisera have given inconsistent and varying proportions of the light and heavy chains12. Springer et al.13 used two-dimensional gel electrophoresis of human B-cell alloantigens to show the association of the two chains, but observed smearing in the isoelectric focusing dimension which they attributed in part to aggregation. We describe here the preliminary successful subfractionation of the 33,000 MW chain of human Ia-like antigens by a modification of the two-dimensional electrophoresis system which combines isoelectric focusing and discontinuous sodium dodecyl sulphate (SDS) electrophoresis. |
| Databáze: | OpenAIRE |
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