Experimental model of intervertebral disc degeneration by needle puncture in Wistar rats
| Autor: | Vitor Castania, E.A. Del Bel, Marcello Henrique Nogueira-Barbosa, M. Castania, Carlos Ernesto Garrido Salmon, Helton Luiz Aparecido Defino, A.C. Issy |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Coccygeal Vertebra Pathology medicine.medical_specialty Time Factors Histology Physiology Radiography Immunology Biophysics Punctures Degeneration (medical) Severity of Illness Index Biochemistry Lesion RATOS WISTAR (EXPERIMENTOS) medicine Animals Fluoroscopy Rats Wistar General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Observer Variation lcsh:R5-920 medicine.diagnostic_test business.industry General Neuroscience Biomedical Sciences Magnetic resonance imaging Intervertebral disc Cell Biology General Medicine Anatomy Magnetic Resonance Imaging Animal models Wistar rats Disease Models Animal medicine.anatomical_structure lcsh:Biology (General) Intervertebral disc degeneration medicine.symptom lcsh:Medicine (General) business |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 46, Iss 3, Pp 235-244 (2013) Brazilian Journal of Medical and Biological Research v.46 n.3 2013 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 3, Pages: 235-244, Published: 15 MAR 2013 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0100-879X |
| DOI: | 10.1590/1414-431x20122429 |
| Popis: | Animal models of intervertebral disc degeneration play an important role in clarifying the physiopathological mechanisms and testing novel therapeutic strategies. The objective of the present study is to describe a simple animal model of disc degeneration involving Wistar rats to be used for research studies. Disc degeneration was confirmed and classified by radiography, magnetic resonance and histological evaluation. Adult male Wistar rats were anesthetized and submitted to percutaneous disc puncture with a 20-gauge needle on levels 6-7 and 8-9 of the coccygeal vertebrae. The needle was inserted into the discs guided by fluoroscopy and its tip was positioned crossing the nucleus pulposus up to the contralateral annulus fibrosus, rotated 360° twice, and held for 30 s. To grade the severity of intervertebral disc degeneration, we measured the intervertebral disc height from radiographic images 7 and 30 days after the injury, and the signal intensity T2-weighted magnetic resonance imaging. Histological analysis was performed with hematoxylin-eosin and collagen fiber orientation using picrosirius red staining and polarized light microscopy. Imaging and histological score analyses revealed significant disc degeneration both 7 and 30 days after the lesion, without deaths or systemic complications. Interobserver histological evaluation showed significant agreement. There was a significant positive correlation between histological score and intervertebral disc height 7 and 30 days after the lesion. We conclude that the tail disc puncture method using Wistar rats is a simple, cost-effective and reproducible model for inducing disc degeneration. |
| Databáze: | OpenAIRE |
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