Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia
| Autor: | Dhaka Ram Bhandari, Søren Thor Larsen, Mette M. Nielsen, Harald S. Hansen, Christian Janfelt, Kate Lykke Lambertsen, Steen Seier Poulsen, Morten Meyer, Bettina Hjelm Clausen, Bernhard Spengler |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Neurons/metabolism Mass Spectrometry Brain Ischemia Mice chemistry.chemical_compound Neurons Arachidonic Acid CD11b Antigen Multidisciplinary Microglia Chemistry Infarction Middle Cerebral Artery Lipidome Biomarkers/chemistry Lipids Lysophosphatidylcholine medicine.anatomical_structure Biochemistry Phospholipases Lysophosphatidylserine Cholesteryl ester CD11b Antigen/metabolism Signal Transduction medicine.medical_specialty Ceramide Docosahexaenoic Acids Lipids/chemistry Article Mass spectrometry imaging Microglia/metabolism 03 medical and health sciences Arachidonic Acid/chemistry Phagocytosis Macrophages/metabolism Brain Ischemia/diagnostic imaging medicine Animals Infarction Middle Cerebral Artery/metabolism Docosahexaenoic Acids/chemistry Inflammation Phospholipases/chemistry Macrophages Enzyme Activation Mice Inbred C57BL Matrix-assisted laser desorption/ionization 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Biomarkers |
| Zdroj: | Scientific Reports Nielsen, M M B, Lambertsen, K L, Clausen, B H, Meyer, M, Bhandari, D R, Larsen, S T, Poulsen, S S, Spengler, B, Janfelt, C & Hansen, H S 2016, ' Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia ', Scientific Reports, vol. 6, 39571 . https://doi.org/10.1038/srep39571 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep39571 |
| Popis: | Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively. |
| Databáze: | OpenAIRE |
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