Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
| Autor: | André César da Silva, Edson Amaro, Antonio J daCosta-Filho, Tatiana Tais Sibov, Ernanni D. Vieira, Lionel Fernel Gamarra, Javier Bustamante Mamani, Lorena Favaro Pavon, Rita Ruiz, W. M. Pontuschka |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Biodistribution Materials science Metabolic Clearance Rate medicine.drug_class ferrofluid Biophysics Contrast Media Pharmaceutical Science Nanoparticle Bioengineering Nanotechnology Monoclonal antibody Epitope Biomaterials Magnetics International Journal of Nanomedicine In vivo Drug Discovery medicine Animals Tissue Distribution Rats Wistar Magnetite Nanoparticles biodistribution Original Research FMR Organic Chemistry Dextrans General Medicine Ferromagnetic resonance Ferrosoferric Oxide quantification In vitro Rats Organ Specificity nanoparticles Quantitative analysis (chemistry) |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s5864 |
| Popis: | Lionel F Gamarra1,2, Antonio J daCosta-Filho3, Javier B Mamani1, Rita de Cassia Ruiz4, Lorena F Pavon1, Tatiana T Sibov1, Ernanni D Vieira3, André C Silva1, Walter M Pontuschka5, Edson Amaro Jr1,21Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil; 2Instituto de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil; 3Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, Brazil; 4Instituto Butantan, São Paulo, Brazil; 5Instituto de Física, Universidade de São Paulo, São Paulo, BrazilAbstract: The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administered in biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the elimination and biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysis of the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carried out in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique for the SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).Keywords: quantification, FMR, ferrofluid, biodistribution, nanoparticles |
| Databáze: | OpenAIRE |
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