Inhibition of bovine retinal microvascular pericyte proliferation in vitro by adenosine
| Autor: | E. C. Carlson, J. A. Jackson |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Adenosine Physiology Biology chemistry.chemical_compound Adenosine A1 receptor Physiology (medical) Internal medicine medicine Animals Cells Cultured Retina Microcirculation Receptors Purinergic Retinal Vessels Retinal Purinergic signalling Adenosine A3 receptor Adenosine receptor Cell biology medicine.anatomical_structure Endocrinology chemistry Cattle Pericyte Cardiology and Cardiovascular Medicine Cell Division medicine.drug |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 263:H634-H640 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1992.263.2.h634 |
| Popis: | Adenosine acts on bovine retinal microvascular pericytes through one or more adenosine receptor subtypes present on the cell surface. Retinal pericytes cultured in medium containing adenosine at concentrations from 10(-6) to 10(-4) M showed significant reduction in proliferation following several days in vitro compared with control cultures. The effects of adenosine were mimicked by polyadenylic acid and inhibited by 8-phenyltheophylline, indicating involvement of a cell surface receptor. Metabolites of adenosine had no effect on pericyte proliferation. An A2 adenosine receptor-specific analogue also inhibited pericyte growth, suggesting that inhibition by adenosine is mediated by A2-receptors and might involve a transient increase in adenosine 3',5'-cyclic monophosphate levels. The results of the present study demonstrate that in addition to demonstrated stimulatory effects on capillary endothelial cells, adenosine also has a direct inhibitory effect on retinal pericytes. We hypothesize a dual function of adenosine within the capillary wall resulting in loss of inhibition of endothelial cells and suggest a role for this nucleoside in pathological neovascularization processes such as proliferative diabetic retinopathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|