Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis
Autor: | Dai Iwase, Kentaro Uchida, Jun Aikawa, Gen Inoue, Shintaro Shoji, Hiroyuki Sekiguchi, Masashi Takaso, Masayuki Miyagi, Shotaro Takano, Manabu Mukai |
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Rok vydání: | 2018 |
Předmět: |
musculoskeletal diseases
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Calcitonin Gene-Related Peptide Prostaglandin E2 Clinical Biochemistry Adipose tissue/PP Neuropeptide Calcitonin gene-related peptide Dinoprostone 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Calcitonin gene-related peptide/SE medicine Humans lcsh:RC620-627 Aged Prostaglandin-E Synthases 030203 arthritis & rheumatology Aged 80 and over Infrapatellar fat pad integumentary system business.industry Research Biochemistry (medical) Stromal vascular fraction Osteoarthritis Knee lcsh:Nutritional diseases. Deficiency diseases Adipose Tissue Gene Expression Regulation Calcitonin Cyclooxygenase 2 Collagenase lipids (amino acids peptides and proteins) business 030217 neurology & neurosurgery medicine.drug Prostaglandin E Signal Transduction |
Zdroj: | Lipids in Health and Disease Lipids in Health and Disease, Vol 17, Iss 1, Pp 1-6 (2018) |
ISSN: | 1476-511X |
Popis: | Background The infrapatellar fat pad (IFP) is implicated in knee osteoarthritis (KOA). Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide expressed in joint tissues and synovial tissues (ST), was recently found to be associated with KOA progression and pain. CGRP is expressed in the IFPs of human KOA patients; however, its regulation has not been elucidated. Methods IFPs and STs were harvested from 138 KOA patients during total knee replacement (TKR) and analyzed for CGRP, cycloxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) expression using real-time polymerase chain reaction (PCR). To investigate CGRP regulation by prostaglandin E2 (PGE2), adipocytes (Ad) and the stromal vascular fraction (SVF) were harvested from IFPs using collagenase. Synovial cells (SYC) were also harvested from ST and stimulated with vehicle (serum-free culture medium), PGE2, or CGRP. Results CGRP, COX-2, and mPGES-1 expression levels were significantly higher in IFPs than STs. PGE2 stimulation increased CGRP expression in Ad, the SVF, and SYC; however, CGRP expression was significantly higher in PGE2-stimulated SVF than PGE2-stimulated SYC. CGRP stimulation had no effect on COX-2 or mPGES-1 expression. Conclusions CGRP expression in the IFP of KOA patients is regulated by the COX-2/mPGES-1/PGE2 pathway. |
Databáze: | OpenAIRE |
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