Regulation of calcitonin gene-related peptide expression through the COX-2/mPGES-1/PGE2 pathway in the infrapatellar fat pad in knee osteoarthritis

Autor: Dai Iwase, Kentaro Uchida, Jun Aikawa, Gen Inoue, Shintaro Shoji, Hiroyuki Sekiguchi, Masashi Takaso, Masayuki Miyagi, Shotaro Takano, Manabu Mukai
Rok vydání: 2018
Předmět:
musculoskeletal diseases
medicine.medical_specialty
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Calcitonin Gene-Related Peptide
Prostaglandin E2
Clinical Biochemistry
Adipose tissue/PP
Neuropeptide
Calcitonin gene-related peptide
Dinoprostone
03 medical and health sciences
0302 clinical medicine
Endocrinology
Internal medicine
Calcitonin gene-related peptide/SE
medicine
Humans
lcsh:RC620-627
Aged
Prostaglandin-E Synthases
030203 arthritis & rheumatology
Aged
80 and over
Infrapatellar fat pad
integumentary system
business.industry
Research
Biochemistry (medical)
Stromal vascular fraction
Osteoarthritis
Knee
lcsh:Nutritional diseases. Deficiency diseases
Adipose Tissue
Gene Expression Regulation
Calcitonin
Cyclooxygenase 2
Collagenase
lipids (amino acids
peptides
and proteins)
business
030217 neurology & neurosurgery
medicine.drug
Prostaglandin E
Signal Transduction
Zdroj: Lipids in Health and Disease
Lipids in Health and Disease, Vol 17, Iss 1, Pp 1-6 (2018)
ISSN: 1476-511X
Popis: Background The infrapatellar fat pad (IFP) is implicated in knee osteoarthritis (KOA). Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide expressed in joint tissues and synovial tissues (ST), was recently found to be associated with KOA progression and pain. CGRP is expressed in the IFPs of human KOA patients; however, its regulation has not been elucidated. Methods IFPs and STs were harvested from 138 KOA patients during total knee replacement (TKR) and analyzed for CGRP, cycloxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) expression using real-time polymerase chain reaction (PCR). To investigate CGRP regulation by prostaglandin E2 (PGE2), adipocytes (Ad) and the stromal vascular fraction (SVF) were harvested from IFPs using collagenase. Synovial cells (SYC) were also harvested from ST and stimulated with vehicle (serum-free culture medium), PGE2, or CGRP. Results CGRP, COX-2, and mPGES-1 expression levels were significantly higher in IFPs than STs. PGE2 stimulation increased CGRP expression in Ad, the SVF, and SYC; however, CGRP expression was significantly higher in PGE2-stimulated SVF than PGE2-stimulated SYC. CGRP stimulation had no effect on COX-2 or mPGES-1 expression. Conclusions CGRP expression in the IFP of KOA patients is regulated by the COX-2/mPGES-1/PGE2 pathway.
Databáze: OpenAIRE
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