Evaluation of Selenite Effects on Selenoproteins and Cytokinome in Human Hepatoma Cell Lines
| Autor: | Francesca Capone, Giovanni Colonna, Maria Grazia Volpe, Melissa Nazzaro, Giuseppe Castello, Eliana Guerriero, Susan Costantini, Biagio Pucci, Fabiola Rusolo, Gianni Di Bernardo, Maria Rita Milone |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
GPX1
Pharmaceutical Science Selenium-Binding Proteins Plasma protein binding Pharmacology Analytical Chemistry Glutathione Peroxidase GPX1 Protein Interaction Mapping Drug Discovery Protein Interaction Maps Selenoproteins interactomics SELK Liver Neoplasms Hep G2 Cells Huh7 Biochemistry Chemistry (miscellaneous) Hepatocellular carcinoma Molecular Medicine medicine.symptom Protein Binding HepG2 Carcinoma Hepatocellular chemistry.chemical_element SELENBP1 Inflammation Biology Article lcsh:QD241-441 Sodium Selenite lcsh:Organic chemistry Cell Line Tumor medicine Carcinoma Humans Physical and Theoretical Chemistry Glutathione Peroxidase Organic Chemistry medicine.disease In vitro cytokines selenite hepatocarcinoma inflammation chemistry Cell culture Hepatocytes Selenium |
| Zdroj: | Molecules, Vol 18, Iss 3, Pp 2549-2562 (2013) Molecules; Volume 18; Issue 3; Pages: 2549-2562 Molecules |
| ISSN: | 1420-3049 |
| Popis: | The need to explore new alternative therapeutic strategies and chemoprevention methods for hepatocellular carcinoma is growing significantly. Selenium is a trace element that plays a critical role in physiological processes, and is used in cancer chemoprevention. The aim of this work was to test in vitro the effect of sodium selenite on the human hepatoma cell lines, HepG2 and Huh7, to assess its effect on the expression of GPX1, SELK and SELENBP1 and also to evaluate its action on inflammation determinants such as cytokines. Our results show that: (i) the increase observed for the GPX1 and SELK expression is correlated with an increase in the sodium selenite concentration, also evidencing an inverse association between the levels of these two proteins and SELENBP1; (ii) the selenium concentrations evaluated in protein extracts increase in proportional way with the selenite concentrations used in the treatment, suggesting that other selenoproteins can also be modulated and should be evaluated in further studies, and (iii) some cytokines, VEGF and three pro-inflammatory cytokines, i.e., IL-6, IL-8, and IL-17, decreased with an increasing selenite concentration. Finally, interactomic studies show that GPX1 and SELK, and the four pro-inflammatory cytokines are functionally correlated evidencing a putative anti-inflammatory role for the selenite. |
| Databáze: | OpenAIRE |
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