Delineation of sites mediating estrogen regulation of the rat creatine kinase B gene
| Autor: | T E Pugliese, H W Dickerman, B T Pentecost, X S Wu-Peng |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
Molecular Sequence Data Response element Estrogen receptor Regulatory Sequences Nucleic Acid Transfection Gene Expression Regulation Enzymologic Endocrinology Gene expression medicine Humans Electrophoresis Gel Two-Dimensional Promoter Regions Genetic Creatine Kinase Molecular Biology Estrogen receptor beta Hormone response element Binding Sites Base Sequence Estradiol biology DNA General Medicine Molecular biology Isoenzymes Receptors Estrogen Regulatory sequence Estrogen biology.protein Creatine kinase HeLa Cells |
| Zdroj: | Molecular Endocrinology. 6:231-240 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/mend.6.2.1569966 |
| Popis: | We have previously confirmed the estrogen-induced protein of rat uterus to be creatine kinase B (CKB), and demonstrated a 1.7-kilobase pair fragment encompassing the promoter and adjoining 5'-flank to be capable of conferring estrogen responsiveness in HeLa cells. In this study we find an element at -550, aGGTCAgaaCACCCt, with limited similarity to the estrogen response element consensus, to be involved in conferring estrogen responsiveness on the CKB promoter. This element can bind estrogen receptor (ER) and is flanked by two GC boxes, which we find capable of binding bacterially expressed Sp1. Additional responsiveness is found closely associated with the CKB promoter at high levels of cotransfected ER construct. No potential response element was identified in this region, but we find the ER DNA-binding domain to be required. |
| Databáze: | OpenAIRE |
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