Novel nucleic acid detection strategies based on CRISPR‐Cas systems: From construction to application
| Autor: | Si-si Xie, Xin-yuan Qiu, Lv-yun Zhu, Chu-shu Zhu, Chuanyang Liu, Lingyun Zhu, Wen-Ying Li |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Computer science Bioengineering Computational biology Polymorphism Single Nucleotide 01 natural sciences Applied Microbiology and Biotechnology Genome 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Animals Humans CRISPR Clustered Regularly Interspaced Short Palindromic Repeats RNA DNA genomic DNA 030104 developmental biology chemistry Nucleic acid CRISPR-Cas Systems Biotechnology Nucleic acid detection |
| Zdroj: | Biotechnology and Bioengineering. 117:2279-2294 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.27334 |
| Popis: | Beyond their widespread application as genome-editing and regulatory tools, clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems also play a critical role in nucleic acid detection due to their high sensitivity and specificity. Recently developed Cas family effectors have opened the door to the development of new strategies for detecting different types of nucleic acids for a variety of purposes. Precise and efficient nucleic acid detection using CRISPR-Cas systems has the potential to advance both basic and applied biological research. In this review, we summarize the CRISPR-Cas systems used for the recognition and detection of specific nucleic acids for different purposes, including the detection of genomic DNA, nongenomic DNA, RNA, and pathogenic microbe genomes. Current challenges and further applications of CRISPR-based detection methods will be discussed according to the most recent developments. |
| Databáze: | OpenAIRE |
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