Membrane mobility and the Concanavalin A binding system of the plasmalemma of higher plant protoplasts

Autor:	P. J. Linstead, J. Burgess
Rok vydání:	1977
Předmět:	Scanning electron microscope fungi food and beverages chemical and pharmacologic phenomena Plant Science Biology Protoplast Crystallography Colloid Membrane Concanavalin A Genetics biology.protein Biophysics Binding site Membrane surface Saturation (chemistry)
Zdroj:	Planta. 136:253-259
ISSN:	1432-2048 0032-0935
DOI:	10.1007/bf00385993
Popis:	The binding of a colloidal gold-Concanavalin A (ConA) complex to the plasmalemma of tobacco leaf protoplasts has been investigated using scanning electron microscopy. At 5° C the particles of gold-ConA appear to be randomly distributed over the surface of the protoplast. If the temperature is raised, the particles associate into clusters. Saturation of the membrane with particles can only occur when the weight of ConA in solution exceeds 1 μg/10(4) protoplasts in suspension, and when its concentration exceeds 15 μg/ml. These results are discussed in terms of the properties of the ConA binding site and the mobility of such sites within the membrane surface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12b89922eb0894e8b4f087d933031056 https://doi.org/10.1007/bf00385993 Zobrazit plný text záznamu Full text from SpringerLink