Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells
| Autor: | Kyoung-mi Bak, Eun Jung Sohn, Hwan Tae Park, Yun-Kyeong Nam |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Curcumin Schwannoma lcsh:RC254-282 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Ribose microRNA Genetics lcsh:QH573-671 Polymerase TUNEL assay biology lcsh:Cytology MicroRNA Transfection lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens RT4 030104 developmental biology Oncology chemistry Apoptosis 030220 oncology & carcinogenesis Cancer research biology.protein Primary Research |
| Zdroj: | Cancer Cell International Cancer Cell International, Vol 18, Iss 1, Pp 1-10 (2018) |
| ISSN: | 1475-2867 |
| Popis: | Background Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were used. A microRNA (miRNA) array was used to identify the miRNAs involved in curcumin-induced apoptosis. To examine miRNA expression, quantitative RT-PCR was used. Results In this study, curcumin exerted cellular cytotoxicity against RT4 schwannoma cells, with an increase in TUNEL-positive cells. Curcumin also activated the expression of apoptotic proteins, such as polyADP ribose polymerase, caspase-3, and caspase-9. The miRNA array revealed that seven miRNAs (miRNA 350, miRNA 17-2-3p, let 7e-3p, miRNA1224, miRNA 466b-1-3p, miRNA 18a-5p, and miRNA 322-5p) were downregulated following treatment with both 10 and 20 μM curcumin in RT4 cells, while four miRNAs (miRNA122-5p, miRNA 3473, miRNA182, and miRNA344a-3p) were upregulated. Interestingly, transfection with a miRNA 344a-3p mimic downregulated the mRNA expression of Bcl2 and upregulated that of Bax, Curcumin treatment in RT 4 cells also reduced the mRNA expression of Bcl2 and enhanced expression of Bax, Overexpression of miRNA344a-3p mimic combined with curcumin treatment activated the expression of apoptotic proteins, including procaspase-9 and cleaved caspase-3 while inhibition of miRNA 344a-3p using miR344a-3p inhibitor repressed cleaved caspase-3 and -9 in curcumin treated RT-4 cells compared to control. Conclusions Our findings demonstrate that curcumin induces apoptosis in schwannoma cells via miRNA 344a-3p. Thus, curcumin may serve as a potent therapeutic agent for the treatment of schwannoma. |
| Databáze: | OpenAIRE |
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