Transcription profiles reveal sugar and hormone signaling pathways mediating tree branch architecture in apple (Malus domestica Borkh.) grafted on different rootstocks

Autor: Li Zhuang, Zhao Deying, Cheng Cungang, Chen Yanhui, An Xiuhong, Renpeng Ma, Li Enmao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Leaves
Cytokinins
Gene Expression
Plant Science
Plant Roots
Biochemistry
01 natural sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Plant Hormones
Abscisic acid
Chromatography
High Pressure Liquid
Plant Proteins
chemistry.chemical_classification
Multidisciplinary
Plant Stems
biology
Plant Biochemistry
Plant Anatomy
Eukaryota
Genomics
Plants
Dwarfing
Horticulture
Phenotype
RNA
Plant
Malus
Cytokinin
Medicine
Malus baccata
Rootstock
Transcriptome Analysis
Signal Transduction
Research Article
Science
Flowers
Fruits
03 medical and health sciences
Auxin
DNA-binding proteins
Genetics
Gene Regulation
Gibberellic acid
Indoleacetic Acids
Apples
Organisms
Biology and Life Sciences
Proteins
Computational Biology
alpha-Glucosidases
Genome Analysis
biology.organism_classification
Gibberellins
Hormones
Regulatory Proteins
Plant Leaves
030104 developmental biology
chemistry
Auxins
Sugars
Abscisic Acid
Transcription Factors
010606 plant biology & botany
Zdroj: PLoS ONE, Vol 15, Iss 7, p e0236530 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Apple trees grafted on different rootstock types, including vigorous rootstock (VR), dwarfing interstock (DIR), and dwarfing self-rootstock (DSR), are widely planted in production, but the molecular determinants of tree branch architecture growth regulation induced by rootstocks are still not well known. In this study, the branch growth phenotypes of three combinations of ‘Fuji’ apple trees grafted on different rootstocks (VR: Malus baccata; DIR: Malus baccata/T337; DSR: T337) were investigated. The VR trees presented the biggest branch architecture. The results showed that the sugar content, sugar metabolism-related enzyme activities, and hormone content all presented obvious differences in the tender leaves and buds of apple trees grafted on these rootstocks. Transcriptomic profiles of the tender leaves adjacent to the top buds allowed us to identify genes that were potentially involved in signaling pathways that mediate the regulatory mechanisms underlying growth differences. In total, 3610 differentially expressed genes (DEGs) were identified through pairwise comparisons. The screened data suggested that sugar metabolism-related genes and complex hormone regulatory networks involved the auxin (IAA), cytokinin (CK), abscisic acid (ABA) and gibberellic acid (GA) pathways, as well as several transcription factors, participated in the complicated growth induction process. Overall, this study provides a framework for analysis of the molecular mechanisms underlying differential tree branch growth of apple trees grafted on different rootstocks.
Databáze: OpenAIRE
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