Inhibition of TGF-β2-induced migration and epithelial-mesenchymal transition in ARPE-19 by sulforaphane
| Autor: | Yang Liu, Hui Zheng, Ye Liu, Ping-Ping Liu, Xiu-Xia Yang, Cheng-Cheng Yang, Yan-Bing Huang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Migration Assay
Stress fiber biology business.industry epithelial-mesenchymal transition sulforaphane Cell migration arpe-19 SMAD RE1-994 Cell biology Fibronectin Ophthalmology Basic Research biology.protein Medicine Phosphorylation Epithelial–mesenchymal transition business Protein kinase B transforming growth factor-β2 |
| Zdroj: | Int J Ophthalmol International Journal of Ophthalmology, Vol 14, Iss 7, Pp 973-980 (2021) |
| ISSN: | 2222-3959 |
| Popis: | AIM: To investigate the effects of sulforaphane (SFN) on transforming growth factor (TGF)-β2 stimulated migration and epithelial-mesenchymal transition (EMT) in ARPE-19 cells. METHODS: ARPE-19 cells were cultured in the presence or absence of SFN or TGF-β2. SFN toxicity was assessed by performing a lactate dehydrogenase assay (LDH) and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays, and cell migration was evaluated by Transwell migration assay. Actin stress fiber formation in ARPE-19 cells was determined using immunofluorescence analysis. Immunoblotting analysis was used to determine fibronectin and α-smooth muscle actin expressions along with the degree of Smad and Akt phosphorylation. RESULTS: SFN inhibited ARPE-19 migration. Additionally, SFN attenuated TGF-β2-induced appearance of actin stress fibers as well as fibronectin and α-smooth muscle actin expressions in these cells. SFN also hindered the TGF-β2-stimulated phosphorylation of Smad2, Smad3, and Akt. SFN showed no cytotoxicity towards ARPE-19 cells. CONCLUSION: SFN inhibits TGF-β2-stimulated migration and EMT in ARPE-19 cells, probably by preventing the establishment of actin stress fibers and Akt and Smad2/3 signaling. |
| Databáze: | OpenAIRE |
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