Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila
| Autor: | Nanako Bowden, Hiroshi Nakato, Masahiko Takemura |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
GAL4/UAS system
biology MARCM fungi Computational biology biology.organism_classification Article carbohydrates (lipids) Drosophila melanogaster Phenotype Proteoglycan Genetic Techniques Gene expression biology.protein Animals Drosophila (subgenus) Gene Function (biology) Heparan Sulfate Proteoglycans |
| Zdroj: | Methods Mol Biol Methods in Molecular Biology ISBN: 9781071613979 |
| ISSN: | 1940-6029 |
| Popis: | Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out. |
| Databáze: | OpenAIRE |
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