| Autor: |
N. Torow, R. Li, T. Hitch, C. Mingels, S. al Bounny, N. van Best, E.-L. Stange, A. Benabid, L. Rüttger, M. Gadermayr, S. Runge, N. Treichel, D. Merhof, S. Rosshart, N. Jehmlich, M. von Bergen, F. Heymann, T. Clavel, F. Tacke, H. Lelouard, I. Costa, M. W. Hornef |
| Rok vydání: |
2022 |
| Zdroj: |
Cold Spring Harbor : Cold Spring Harbor Laboratory, bioRxiv : the preprint server for biology 1-41 (2022). doi:10.1101/2022.03.03.482839 |
| DOI: |
10.1101/2022.03.03.482839 |
| Popis: |
Marked differences exist between the mucosal immune system of the neonate and adult host. The pronounced influence of the enteric microbiota in adults suggests a causal relationship between postnatal colonization and immune maturation. However, using metagenomic, metaproteomic, and functional immunological analyses we demonstrate an early presence of bacteria and immunogenic microbial antigens preceding immune maturation in the small intestine, the primary inductive site of intestinal immunity. Instead, transcriptomic, flow cytometric and histological analysis indicated neonatal Peyer’s patch (PP) mononuclear phagocytes (MNP) as rate limiting factor of postnatal immune maturation. Despite the early presence of MNPs, conventional dendritic cells (cDC) of type 1, 2a and 2b exhibited significant age-dependent differences in tissue distribution and cellular composition. Single cell transcriptional profiling and functional assays revealed decreased antimicrobial and antigen processing/presentation capacity, an overall retarded cell maturation and reduced antigen uptake. In cDC2a this resulted in a reduced proportion of CCR7+migratory cells and a consequent defect in CD4 T cell priming. Interestingly, transcriptional profiling of neonatal DC subsets identified reduced expression of type I interferon (IFN)-stimulated genes (ISG). Type I IFN induction by oral administration of the TLR7 agonist R848 accelerated MNP maturation and enhanced cognate antigen CD4 T cell priming. However, humoral responses to oral vaccination in the presence of R848 were significantly reduced. Together, our results identify PP MNP maturation as pacemaker of postnatal mucosal immune priming, indicate the biological role of delayed maturation and demonstrate that targeted interventional strategies allow manipulation of mucosal responses in early life. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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