PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element
| Autor: | Sanghoon Lee, Boris Bartholdy, Constanze Bonifer, Florence Guibal, Maarten Hoogenkamp, Tajhal Dayaram, Pu Zhang, Johanna Klupp, Britta Will, Meritxell Alberich Jordà, Frank Rosenbauer, Katherine L. B. Borden, Alexander K. Ebralidze, Victoria Petkova, Daniel G. Tenen, Gang Huang, Karen B. O'Brien, Ulrich Steidl |
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| Rok vydání: | 2008 |
| Předmět: |
Transcription
Genetic T-Lymphocytes Regulator Gene Expression HL-60 Cells Regulatory Sequences Nucleic Acid Biology Cell Line Jurkat Cells Mice RNA interference Proto-Oncogene Proteins Sense (molecular biology) Gene expression Genetics Animals Humans RNA Antisense RNA Messenger RNA Small Interfering Small nucleolar RNA Gene Transcription factor Models Genetic Immunomagnetic Separation Macrophages U937 Cells Long non-coding RNA Cell biology Electroporation Protein Biosynthesis Trans-Activators RNA Interference Granulocytes Research Paper Developmental Biology |
| Zdroj: | Genes & Development. 22:2085-2092 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.1654808 |
| Popis: | The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function. |
| Databáze: | OpenAIRE |
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