Nosocomial, Multidrug-ResistantKlebsiella pneumoniaeStrains Isolated from Mexico City Produce Robust Biofilms on Abiotic Surfaces but Not on Human Lung Cells
| Autor: | Martha Lorena Ostria-Hernandez, Jorge E. Vidal, Fuminori Sakai, Karla Cecilia Juárez-de la Rosa, Graciela Castro-Escarpulli, Patricia Arzate-Barbosa, J. Antonio Ibarra, Antonino Lara-Hernández |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Epidemiology Klebsiella pneumoniae medicine.drug_class 030106 microbiology Immunology Fimbria Antibiotics Virulence Microbiology 03 medical and health sciences Antibiotic resistance Bacterial Proteins Enterobacteriaceae Drug Resistance Multiple Bacterial medicine Humans Adhesins Bacterial Mexico Cells Cultured Pharmacology Cross Infection biology Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Hospitals Anti-Bacterial Agents Klebsiella Infections Bacterial adhesin Multiple drug resistance Carbapenems Biofilms Fimbriae Bacterial |
| Zdroj: | Microbial Drug Resistance. 24:422-433 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2017.0073 |
| Popis: | Background: Klebsiella pneumoniae (Kpn) strains are a leading cause of hospital-acquired infections, including ventilator-associated pneumonia. Resistance to antibiotics, biofilm formation, and the production of certain fimbriae play an important role in the pathogenesis. Aim: We investigated the genetic relatedness, antibiotic resistance, virulence potential, and ability to form biofilms of Kpn strains isolated from hospital-acquired infections (n = 76). Strains were isolated at three major hospitals serving the largest metropolitan urban area in Mexico City, Mexico. Results: Enterobacterial repetitive intergenic consensus (ERIC)–PCR demonstrated that clonal groups predominate in each hospital. Selected strains chosen from clonal groups (n = 47) were multidrug resistant (MDR, 83%), although the majority (∼70%) were susceptible to carbapenems. All strains produced robust biofilms on abiotic surfaces, and ∼90% harbored adhesin genes fimH, mrkA, and ecpA. The ultrastructure of biofilms was further studied by high-resolution confocal microscopy. The average height of Kpn biofilms on abiotic surfaces was ∼40 μm. We then assessed formation of biofilms on human lung cells, as a surrogate of lung infection. While Kpn strains formed robust biofilms on abiotic surfaces, studies on lung cells revealed attachment to human cells but scarce formation of biofilms. Gene expression studies revealed a differential temporal expression of an adhesin (ecpA) and a capsule (galF) gene when biofilms were formed on different substrates. Conclusions: Kpn strains isolated from nosocomial infections in Mexico City are MDR, although the majority are still susceptible to carbapenems and form more robust biofilms on polystyrene in comparison to those formed on human cells. |
| Databáze: | OpenAIRE |
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