Evaluation and Optimization of Poly-D-Lysine as a Non-Natural Cationic Polypeptide for Gene Transfer in Neuroblastoma Cells
| Autor: | Gema Martínez-Navarrete, Eduardo Fernández, Miguel Sanchez-Martos, Adela Bernabeu-Zornoza, Lawrence Humphreys |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
SH-SY5Y General Chemical Engineering Genetic enhancement Gene delivery PDL 3T3 Article Flow cytometry Green fluorescent protein HeLa 03 medical and health sciences neuroblastoma 0302 clinical medicine medicine General Materials Science QD1-999 biology medicine.diagnostic_test Chemistry nonviral vector poly-lysine Transfection biology.organism_classification gene therapy Cell biology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis CPP |
| Zdroj: | Nanomaterials Volume 11 Issue 7 Nanomaterials, Vol 11, Iss 1756, p 1756 (2021) r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante instname |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11071756 |
| Popis: | Cationic polypeptides and cationic polymers have cell-penetrating capacities and have been used in gene transfer studies. In this study, we investigate the capability of a polymer of d-lysine (PDL), a chiral form of α–Poly-lysine, as a possible nonviral vector for releasing genetic materials to neuroblastoma cells and evaluate its stability against proteases. We tested and compared its transfection effectiveness in vitro as a vehicle for the EGFP plasmid DNA (pDNA) reporter in the SH-SY5Y human neuroblastoma, HeLa, and 3T3 cell lines. Using fluorescent microscopy and flow cytometry, we demonstrated high transfection efficiencies based on EGFP fluorescence in SH-SY5Y cells, compared with HeLa and 3T3. Our results reveal PDL as an efficient vector for gene delivery specifically in the SH-SY5Y cell line and suggest that PDL can be used as a synthetic cell-penetrating polypeptide for gene therapy in neuroblastoma cells. |
| Databáze: | OpenAIRE |
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