Recovery of ovarian function by human embryonic stem cell-derived mesenchymal stem cells in cisplatin-induced premature ovarian failure in mice

Autor: Sang Woo Lyu, Eun-Young Shin, Jin Hee Eum, Mira Park, Dong Ryul Lee, Haengseok Song, Sook Young Yoon, Jeoung Eun Lee, Jung Ah Yoon, Sookyung Jung, Woo Sik Lee
Jazyk: angličtina
Rok vydání: 2020
Předmět:
media_common.quotation_subject
Population
Human Embryonic Stem Cells
Chemotherapy-induced premature ovarian failure
Medicine (miscellaneous)
Ovarian stromal cell apoptosis
Ovary
Primary Ovarian Insufficiency
Mesenchymal Stem Cell Transplantation
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Andrology
lcsh:Biochemistry
Mice
medicine
Human embryonic stem cell-derived MSC
Animals
Humans
lcsh:QD415-436
education
Ovulation
reproductive and urinary physiology
media_common
education.field_of_study
lcsh:R5-920
business.industry
Research
Mesenchymal stem cell
Recovery of ovarian function
Mesenchymal Stem Cells
Cell Biology
medicine.disease
Premature ovarian failure
Transplantation
medicine.anatomical_structure
embryonic structures
Molecular Medicine
Female
Folliculogenesis
Stem cell
Cisplatin
business
lcsh:Medicine (General)
Zdroj: Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-13 (2020)
Stem Cell Research & Therapy
ISSN: 1757-6512
DOI: 10.1186/s13287-020-01769-6
Popis: Background Clinical use of mesenchymal stem cells (MSCs) requires a uniform cell population, and their harvesting is invasive and produces a limited number of cells. Human embryonic stem cell-derived MSCs (hESC-MSCs) can differentiate into three germ layers and possess immunosuppressive effects in vitro. Anticancer treatment is a well-known risk factor for premature ovarian failure (POF). In this study, we investigated the effect of hESC-MSC on recovery of ovarian function in cisplatin-induced POF in mice. Methods Female mice received intraperitoneal cisplatin for 10 days. On day 12, CHA15-derived hESC-MSCs were transplanted into the mice by tail vein injection. An injection of PBS served as the negative control. Ovaries were removed 28 days after transplantation for assessment of ovarian histology, immunostaining, and fertility testing by superovulation and in vitro fertilization. hESC-MSC transplantation into mice with cisplatin-induced damage restored body weight and ovary size. Results Mean primary and primordial follicle counts in the hESC-MSC group were significantly improved compared to the PBS group (P P Conclusions hESC-MSC restored structure and function in the cisplatin-damaged ovary. Our study provides new insights into the great clinical potential of human hESC-MSC in treating POF.
Databáze: OpenAIRE
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