Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast Cyberlindnera jadinii

Autor: Maria Sousa-Silva, Pedro Soares, João Alves, Daniel Vieira, Margarida Casal, Isabel Soares-Silva
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Fungi; Volume 8; Issue 1; Pages: 51
Journal of Fungi
Journal of Fungi, Vol 8, Iss 51, p 51 (2022)
ISSN: 2309-608X
DOI: 10.3390/jof8010051
Popis: The yeast Cyberlindnera jadinii has great potential in the biotechnology industry due to its ability to produce a variety of compounds of interest, including carboxylic acids. In this work, we identified genes encoding carboxylate transporters from this yeast species. The functional characterization of sixteen plasma membrane carboxylate transporters belonging to the AceTr, SHS, TDT, MCT, SSS, and DASS families was performed by heterologous expression in Saccharomyces cerevisiae. The newly identified C. jadinii transporters present specificity for mono-, di-, and tricarboxylates. The transporters CjAto5, CjJen6, CjSlc5, and CjSlc13-1 display the broadest substrate specificity; CjAto2 accepts mono- and dicarboxylates; and CjAto1,3,4, CjJen1-5, CjSlc16, and CjSlc13-2 are specific for monocarboxylic acids. A detailed characterization of these transporters, including phylogenetic reconstruction, 3D structure prediction, and molecular docking analysis is presented here. The properties presented by these transporters make them interesting targets to be explored as organic acid exporters in microbial cell factories.
Databáze: OpenAIRE
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