Transcriptomic Profile Reveals Deregulation of Hearing-Loss Related Genes in Vestibular Schwannoma Cells Following Electromagnetic Field Exposure

Autor: Valentina Melfi, Peter A. Greer, Adam J Reid, Valerio Magnaghi, Nico Mitro, Alessandra Colciago, Veronica Bonalume, Tasnim Mohamed, Alessandro Faroni, Matteo Audano
Rok vydání: 2021
Předmět:
0301 basic medicine
Neuroma
Acoustic/etiology
Schwannoma
Transcriptome
0302 clinical medicine
Cell Movement
Neurofilament Proteins
Biology (General)
Electromagnetic Fields/adverse effects
Vestibular system
Differential display
REST
Schwann Cells/metabolism
Neuroma
Acoustic
General Medicine
Schwann cell
GJB2
Connexin 26
NEFL
medicine.anatomical_structure
030220 oncology & carcinogenesis
Proteins/genetics
Connexin 26/genetics
TPRN
medicine.symptom
Intracellular
Signal Transduction
animal structures
Cell Survival
QH301-705.5
Hearing loss
Primary Cell Culture
Cell Proliferation/radiation effects
Biology
Article
03 medical and health sciences
Cell Movement/radiation effects
Electromagnetic Fields
Neurofilament Proteins/genetics
medicine
Humans
Membrane Proteins/genetics
Cell Proliferation
hearing loss
Hearing Loss/etiology
Repressor Proteins/genetics
Cell growth
Gene Expression Profiling
Cell Survival/radiation effects
OTOGL
Membrane Proteins
Proteins
medicine.disease
Repressor Proteins
030104 developmental biology
Gene Expression Regulation
NF2
Cancer research
Schwann Cells
Zdroj: Cells, Vol 10, Iss 1840, p 1840 (2021)
Cells
Volume 10
Issue 7
Colciago, A, Audano, M, Bonalume, V, Melfi, V, Mohamed, T, Reid, A J, Faroni, A, Greer, P A, Mitro, N & Magnaghi, V 2021, ' Transcriptomic Profile Reveals Deregulation of Hearing-Loss Related Genes in Vestibular Schwannoma Cells Following Electromagnetic Field Exposure ', Cells, vol. 10, no. 7, 1840 . https://doi.org/10.3390/cells10071840
ISSN: 2073-4409
DOI: 10.3390/cells10071840
Popis: Hearing loss (HL) is the most common sensory disorder in the world population. One common cause of HL is the presence of vestibular schwannoma (VS), a benign tumor of the VIII cranial nerve, arising from Schwann cell (SC) transformation. In the last decade, the increasing incidence of VS has been correlated to electromagnetic field (EMF) exposure, which might be considered a pathogenic cause of VS development and HL. Here, we explore the molecular mechanisms underlying the biologic changes of human SCs and/or their oncogenic transformation following EMF exposure. Through NGS technology and RNA-Seq transcriptomic analysis, we investigated the genomic profile and the differential display of HL-related genes after chronic EMF. We found that chronic EMF exposure modified the cell proliferation, in parallel with intracellular signaling and metabolic pathways changes, mostly related to translation and mitochondrial activities. Importantly, the expression of HL-related genes such as NEFL, TPRN, OTOGL, GJB2, and REST appeared to be deregulated in chronic EMF exposure. In conclusion, we suggest that, at a preclinical stage, EMF exposure might promote the transformation of VS cells and contribute to HL.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje