Cell-type–specific transcriptome and histone modification dynamics during cellular reprogramming in the Arabidopsis stomatal lineage
| Autor: | Diego L. Wengier, Laura R. Lee, Dominique C. Bergmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Cell type Callus formation H3K27me3 stomata Arabidopsis Plant Biology macromolecular substances 01 natural sciences REPROGRAMMING Ciencias Biológicas purl.org/becyt/ford/1 [https] Transcriptome 03 medical and health sciences Guard cell purl.org/becyt/ford/1.6 [https] Ciencias de las Plantas Botánica 030304 developmental biology 0303 health sciences Multidisciplinary biology fungi food and beverages reprogramming Biological Sciences ARABIDOPSIS biology.organism_classification STOMATA Cell biology Histone biology.protein H3K4me3 Reprogramming CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Significance Houseplant enthusiasts know the power of plant regeneration—small cuttings can regrow into whole plants. Yet normally, plant tissues and cells maintain distinct and stable identities. A biochemical modification to histones (H3K27me3) is thought to lock in cell identity by preventing access to specific regions of DNA. Here, we investigated how H3K27me3 was used to maintain the identity of stomatal guard cells. We identified changes in H3K27me3 distribution and gene expression in normal guard cells and after we induced “reprogramming” into alternative cell identities. This linked the loss of H3K27me3 at stomatal precursor genes to a return to an earlier fate, but increased H3K27me3 at a wound-induced reprogramming gene indicated how plant cells may sense and resist inappropriate loss of identity. Plant cells maintain remarkable developmental plasticity, allowing them to clonally reproduce and to repair tissues following wounding; yet plant cells normally stably maintain consistent identities. Although this capacity was recognized long ago, our mechanistic understanding of the establishment, maintenance, and erasure of cellular identities in plants remains limited. Here, we develop a cell-type–specific reprogramming system that can be probed at the genome-wide scale for alterations in gene expression and histone modifications. We show that relationships among H3K27me3, H3K4me3, and gene expression in single cell types mirror trends from complex tissue, and that H3K27me3 dynamics regulate guard cell identity. Further, upon initiation of reprogramming, guard cells induce H3K27me3-mediated repression of a regulator of wound-induced callus formation, suggesting that cells in intact tissues may have mechanisms to sense and resist inappropriate dedifferentiation. The matched ChIP-sequencing (seq) and RNA-seq datasets created for this analysis also serve as a resource enabling inquiries into the dynamic and global-scale distribution of histone modifications in single cell types in plants. |
| Databáze: | OpenAIRE |
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