Involvement of glutathione transferases, Gtt1and Gtt2, with oxidative stress response generated by H2O2during growth ofSaccharomyces cerevisiae
| Autor: | Cristiane Juliano Mathias, Anita D. Panek, Ricardo Nuno Correia Pereira, Diana Mariani, Carmelita Gomes da Silva, Elis C. A. Eleutherio, R.S. Herdeiro, Marcos D. Pereira |
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| Rok vydání: | 2008 |
| Předmět: |
GPX1
Saccharomyces cerevisiae Proteins GPX3 Physiology Clinical Biochemistry Glutathione reductase Saccharomyces cerevisiae Glucosephosphate Dehydrogenase Biology GPX4 Protein oxidation medicine.disease_cause Biochemistry GPX6 Protein Carbonylation chemistry.chemical_compound medicine Glutathione Transferase Biochemistry (medical) Hydrogen Peroxide Cell Biology Glutathione Oxidants Molecular biology Isoenzymes Oxidative Stress Glutathione Reductase chemistry Mutation Lipid Peroxidation Oxidative stress |
| Zdroj: | Redox Report. 13:246-254 |
| ISSN: | 1743-2928 1351-0002 |
| DOI: | 10.1179/135100008x309028 |
| Popis: | Glutathione transferases are detoxifying enzymes responsible for eliminating toxic compounds generated under a variety of stress conditions. Saccharomyces cerevisiae control cells and glutathione transferase mutant strains (gtt1 and gtt2) were used to analyze tolerance, lipid and protein oxidation as oxidative stress markers during growth in the presence of H2O2. Glucose 6-phosphate dehydrogenase (G6PD) and glutathione reductase were assayed to monitor the capacity of cells to recycle glutathione. Although a reduction in growth was observed, deletion of GTT1 showed less inhibition by H2O2 than the control strain. Cells showed a significant reduction in cellular viability during the first hours of growth, the gtt1 mutant being hypersensitive even after 24 h of H2O2 exposure. As a consequence of oxidative stress caused by exposure to H2O2, an increase in lipid peroxidation was observed, mainly in the glutathione transferase mutant strains. While protein carbonylation increased by 17% and 23%, respectively, after 2 h in the presence of H2O2 in the control and gtt2 mutant, a 40% increase was observed in the gtt1 strain after 24-h exposure. The antioxidant G6PD and glutathione reductase activities were affected in the gtt1 mutant during H2O2 exposure, which could be critical for recycling glutathione. The same was observed for the gtt2 mutant after 2-h treatment, indicating that glutathione recycling might be associated with the detoxification process. Thus, glutathione transferases, Gtt1 and Gtt2, seem to be crucial in the response to H2O2 stress. |
| Databáze: | OpenAIRE |
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