Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau†
| Autor: | Vladimir Milojevic, Alba Rudolf Vegas, Vera A. van der Weijden, Susanne E. Ulbrich, Corina I. Schanzenbach, Barbara Drews, Brina Puntar, Mariusz P. Kowalewski |
|---|---|
| Přispěvatelé: | University of Zurich, Ulbrich, Susanne E |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
10077 Institute of Veterinary Anatomy Embryonic Development Pregnancy Proteins Biology Endometrium 1307 Cell Biology Embryo Culture Techniques Andrology 03 medical and health sciences 0302 clinical medicine biology.animal Progesterone receptor medicine Animals 030219 obstetrics & reproductive medicine preimplantation embryo Deer Embryogenesis Placentation diapause endometrium Epithelial Cells Embryo Cell Biology General Medicine 2743 Reproductive Medicine Embryo Mammalian Interferon tau Roe deer 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine Interferon Type I 570 Life sciences biology Female Retinol binding |
| Zdroj: | Biology of Reproduction, 101 (5) |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/ioz129 |
| Popis: | Numerous intrauterine changes take place across species during embryo development. Following fertilization in July/August, the European roe deer (Capreolus capreolus) embryo undergoes diapause until embryonic elongation in December/January. Embryonic elongation prior to implantation is a common feature among ungulates. Unlike many other ruminants, the roe deer embryo does not secrete interferon-tau (IFNτ). This provides the unique opportunity to unravel IFNτ-independent signaling pathways associated with maternal recognition of pregnancy (MRP). This study aimed at identifying the cell-type–specific endometrial gene expression changes associated with the MRP at the time of embryo elongation that are independent of IFNτ in roe deer. The messenger RNA (mRNA) expression of genes known to be involved in embryo–maternal communication in cattle, pig, sheep, and mice was analyzed in laser capture microdissected (LMD) endometrial luminal, glandular epithelial, as well as stromal cells. The mRNA transcript abundances of the estrogen (ESR1), progesterone receptor (PGR), and IFNτ-stimulated genes were lower in the luminal epithelium in the presence of an elongated embryo compared to diapause. Retinol Binding Protein-4 (RBP4), a key factor involved in placentation, was more abundant in the luminal epithelium in the presence of an elongated embryo. The progesterone receptor localization was visualized by immunohistochemistry, showing an absence in the luminal epithelium and an overall lower abundance with time and thus prolonged progesterone exposure. Our data show a developmental stage-specific mRNA expression pattern in the luminal epithelium, indicating that these cells sense the presence of an elongated embryo in an IFNτ-independent manner. Biology of Reproduction, 101 (5) ISSN:0006-3363 ISSN:1529-7268 |
| Databáze: | OpenAIRE |
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