HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive
| Autor: | Erik Jakobsen, Dorte Aalund Olsen, Susanne Bokmand, Peter Wamberg, Ivan Brandslund, Birthe Østergaard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Receptor erbB-2 Receptor ErbB-2 Concordance Clinical Biochemistry Breast Neoplasms Enzyme-Linked Immunosorbent Assay Biology Antibodies Monoclonal Humanized Sensitivity and Specificity Breast cancer Trastuzumab Cell Line Tumor medicine Humans In Situ Hybridization Fluorescence medicine.diagnostic_test Biochemistry (medical) Cancer Antibodies Monoclonal General Medicine medicine.disease Immunohistochemistry Neoplasm Proteins Molecular Diagnostic Techniques Female Breast cancer cells HER-2 protein Fluorescence in situ hybridization medicine.drug |
| Zdroj: | Olsen, D A, Østergaard, B, Bokmand, S, Wamberg, P A, Jakobsen, E H & Brandslund, I 2007, ' HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive ', Clinical Chemistry and Laboratory Medicine, vol. 45, no. 2, pp. 177-182 . https://doi.org/10.1515/CCLM.2007.034 |
| Popis: | BACKGROUND: The level of HER-2/neu in breast cancer cells is normally measured by immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH). It determines whether patients should be treated with trastuzumab (Herceptin). In this study, HER-2 protein in breast cancer tissue was measured using a quantitative method. METHODS: Tissue samples of malignant and adjacent benign breast tissue were collected from 118 consecutive women admitted for surgical treatment of breast cancer. The HER-2 protein concentration was determined by 2 HER-2 assays: ELISA and the Bayer ADVIA Centaur assay. Paraffin-embedded tissue sections of the corresponding tumors were analyzed by IHC and FISH. RESULTS: Increased HER-2 concentrations in cancer tissue were found compared to autologous reference tissue (p |
| Databáze: | OpenAIRE |
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