Comprehensive Glycomic Approach Reveals Novel Low-Molecular-Weight Blood Group-Specific Glycans in Serum and Cerebrospinal Fluid
| Autor: | Hisatoshi Hanamatsu, Tomohiro Ando, Yasuro Shinohara, Megumi Hirayama, Yasuyuki Igarashi, Jun-ichi Furukawa, Shota Sakai, Naoya Sakamoto, Shunsuke Ohnishi, Kazue Okada, Hiroyuki Kobayashi, Kohei Yuyama, Nobuaki Miura, Ikuko Yokota, Makoto Ito |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Glycan Glycoconjugate Oligosaccharides Biochemistry 03 medical and health sciences Cerebrospinal fluid Polysaccharides ABO blood group system medicine Humans Glycomics Glycoproteins chemistry.chemical_classification Blood type 030102 biochemistry & molecular biology biology Chemistry General Chemistry Red blood cell 030104 developmental biology medicine.anatomical_structure biology.protein Blood Group Antigens Antibody Glycoprotein |
| Zdroj: | Journal of proteome research. 20(5) |
| ISSN: | 1535-3907 |
| Popis: | ABO blood antigens on the human red blood cell membrane as well as different cells in various human tissues have been thoroughly studied. Anti-A and -B antibodies of IgM are present in serum/plasma, but blood group-specific glyco-antigens have not been extensively described. In this study, we performed comprehensive and quantitative serum glycomic analyses of various glycoconjugates and free oligosaccharides in all blood groups. Our comprehensive glycomic approach revealed that blood group-specific antigens in serum/plasma are predominantly present on glycosphingolipids on lipoproteins rather than glycoproteins. Expression of the ABO antigens on glycosphingolipids depends not only on blood type but also on secretor status. Blood group-specific glycans in serum/plasma were classified as type I, whereas those on RBCs had different structures including hexose and hexosamine residues. Analysis of free oligosaccharides revealed that low-molecular-weight blood group-specific glycans, commonly containing lacto-N-difucotetraose, were expressed in serum/plasma according to blood group. Furthermore, comprehensive glycomic analysis in human cerebrospinal fluid showed that many kinds of free oligosaccharides were highly expressed, and low-molecular-weight blood group-specific glycans, which existed in plasma from the same individuals, were present. Our findings provide the first evidence for low-molecular-weight blood group-specific glycans in both serum/plasma and cerebrospinal fluid. |
| Databáze: | OpenAIRE |
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