Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)
| Autor: | Chao Qi, Gongqing Feng, Hao-Bin Zhao, Xueyan Zhang, Qingchun Zhou, Jundan Duan, Songguang Xie, Xueping Zhong, Pei Chang |
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| Rok vydání: | 2014 |
| Předmět: |
endocrine system
Physiology Molecular Sequence Data Cyprinidae Aquatic Science Biology Biochemistry biology.animal Gene expression medicine Animals Cluster Analysis Amino Acid Sequence Cloning Molecular Zebrafish Gene In Situ Hybridization Phylogeny DNA Primers Genetics Regulation of gene expression Likelihood Functions Base Sequence Models Genetic Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Embryogenesis Computational Biology Gene Expression Regulation Developmental RNA-Binding Proteins General Medicine Sequence Analysis DNA Minnow Zebrafish Proteins biology.organism_classification Cell biology medicine.anatomical_structure Germ Cells Gobiocypris rarus Sequence Alignment Germ cell |
| Zdroj: | Fish physiology and biochemistry. 41(2) |
| ISSN: | 1573-5168 |
| Popis: | Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow. |
| Databáze: | OpenAIRE |
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