High-resolution imaging reveals how the spindle midzone impacts chromosome movement
| Autor: | Lina Carlini, Tarun M. Kapoor, Melissa C. Pamula, Priyanka Verma, Scott Forth, Subbulakshmi Suresh, Wesley R. Legant, Alexey Khodjakov, Eric Betzig |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chromosome movement
Movement Cell Cycle Proteins Spindle Apparatus macromolecular substances Biology Microtubules Article Spindle elongation Protein filament Chromosome segregation 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine Microtubule Chromosome Segregation Chromosomes Human Humans Research Articles 030304 developmental biology Anaphase 0303 health sciences Spindle midzone Cell Biology Cell biology Mutation Microtubule bundle Microtubule-Associated Proteins 030217 neurology & neurosurgery Fluorescence Recovery After Photobleaching HeLa Cells |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201904169 |
| Popis: | Microtubule bundles in the spindle midzone have been reported to either promote or hinder chromosome movement. Pamula et al. examine the assembly dynamics of midzone microtubule bundles during anaphase and how chromosome segregation is impacted by aberrant bundle assembly. In the spindle midzone, microtubules from opposite half-spindles form bundles between segregating chromosomes. Microtubule bundles can either push or restrict chromosome movement during anaphase in different cellular contexts, but how these activities are achieved remains poorly understood. Here, we use high-resolution live-cell imaging to analyze individual microtubule bundles, growing filaments, and chromosome movement in dividing human cells. Within bundles, filament overlap length marked by the cross-linking protein PRC1 decreases during anaphase as chromosome segregation slows. Filament ends within microtubule bundles appear capped despite dynamic PRC1 turnover and submicrometer proximity to growing microtubules. Chromosome segregation distance and rate are increased in two human cell lines when microtubule bundle assembly is prevented via PRC1 knockdown. Upon expressing a mutant PRC1 with reduced microtubule affinity, bundles assemble but chromosome hypersegregation is still observed. We propose that microtubule overlap length reduction, typically linked to pushing forces generated within filament bundles, is needed to properly restrict spindle elongation and position chromosomes within daughter cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|