Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital
Autor: | Roberto Martinez, Heliara Maria Spina Canela, Miliane Rodrigues Frazão, Lúcia Helena Vitali, Márcia Eliana da Silva Ferreira, Bárbara Rita Cardoso, Juliana Pfrimer Falcão |
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Rok vydání: | 2021 |
Předmět: |
Adult
Male Adolescent FILOGENIA Bacterial Fungal and Virus Molecular Biology - Research Paper Microbiology Candida tropicalis Young Adult 03 medical and health sciences Genotype Media Technology Pulsed-field gel electrophoresis Humans Genetic variability Child Mycological Typing Techniques Candida albicans Phylogeny Aged Candida 030304 developmental biology Aged 80 and over 0303 health sciences Genetic diversity Candida glabrata biology 030306 microbiology Infant Newborn Candidemia Genetic Variation Infant Middle Aged bacterial infections and mycoses biology.organism_classification Hospitals Electrophoresis Gel Pulsed-Field Child Preschool Multilocus sequence typing Female Brazil Multilocus Sequence Typing |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Braz J Microbiol |
Popis: | Candida spp. are the main causative agents of invasive fungal infections in immunocompromised patients. Candidemia has attributable mortality rates of 15 to 35% and increases hospitalisation time and costs, thus making this disease a public health concern. This study aimed to use pulsed-field gel electrophoresis (PFGE), microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST) to analyse the genetic relationships among 65 Candida spp. bloodstream isolates, including 35 Candida albicans, 15 Candida glabrata and 15 Candida tropicalis isolates, all of which were obtained from patients in a Brazilian hospital. Moreover, patient clinical data were assessed. All techniques resulted in high discriminatory indexes. C. albicans and C. tropicalis isolates showed high genetic variability, while C. glabrata isolates had relatively low genetic variability. Moreover, a cluster of C. glabrata isolates was identified in a hospital unit. New MLST sequence types, diploid sequence types and alleles are described. Relationships were not observed between the molecular typing results and clinical characteristics. The molecular typing of clinical strains increases our understanding of candidemia epidemiology and promotes the development of strategies that can reduce the incidence of this disease. Moreover, this study is the first to combine these techniques to genotype these three species in Brazil. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00446-x. |
Databáze: | OpenAIRE |
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