Antiproliferative Effect of a Prostatic Cell-Derived Activity on the Human Androgen-Dependent Prostatic Carcinoma Cell Line LNCaP
| Autor: | J. W. Chiao, Mohsen Abolhassani |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty RNase P T cell Immunology Cell urologic and male genital diseases Ribonucleases Neutralization Tests Virology Internal medicine Endopeptidases LNCaP Tumor Cells Cultured medicine Humans S phase Deoxyribonucleases Cell growth Chemistry Carcinoma Cell Cycle Temperature Prostatic Neoplasms Cell Biology Hydrogen-Ion Concentration Dithiothreitol medicine.anatomical_structure Endocrinology Apoptosis Culture Media Conditioned Androgens Cancer research Cytokines Female Cell Division Promyelocyte |
| Zdroj: | Journal of Interferon & Cytokine Research. 15:179-185 |
| ISSN: | 1557-7465 1079-9907 |
| Popis: | We have identified a new antiproliferative activity from the conditioned medium of two androgen-independent prostatic cancer cell lines, PC3 and DU-145. This antiproliferative activity selectively inhibited cell proliferation of an androgen-dependent prostate cancer cell line LNCaP in a dose-dependent manner. No antiproliferative activity was observed against mouse fibroblast 3T3, normal human lymphocytes, human leukemic cells, including promyelocyte HL-60 or T cell HUT-78, or human adenocarcinoma cell lines, including prostatic cells JCA-1, ovary NIH:OVCAR-3, cervix C-33A, or breast MDA-MB-231. Cell cycle analysis revealed that the antiproliferative activity did not induce apoptosis in LNCaP cells, but it prevented some G1 LNCaP cells from entering into the S phase of the cell cycle. The antiproliferative activity was sensitive to high temperature (100 degrees C) and to proteinase digestion; however, it was resistant to 56 degrees C, pH 2.0, and reducing agent treatment, as well as to DNase and RNase digestion. The antiproliferative activity was partially purified by gel filtration, ion-exchange chromatography, and SDS-PAGE, with an apparent molecular weight of 50 kD. The antiproliferative activity was not affected by neutralizing antibody against TGF-beta 1,2,3, TNF-alpha, PDGF, EGF, IL-1, IL-2, IL-3, IL-4, or IL-6. |
| Databáze: | OpenAIRE |
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