Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor
Autor: | Megha Padi, Anna Korkhin, James A. DeCaprio, Robert L. James, Michael A. Calderwood, Rosa Yoon, Jarrod A. Marto, Marc Vidal, Luxuan Guo, Christian Berrios, Ying Zhang, Jingwei Cheng, Soundarapandian Velmurgan, Laurence Florens, Guillaume Adelmant, Michael E. Cusick, Debrah A. Fine, Michael P. Washburn, David E. Hill, Orit Rozenblatt-Rosen, Larisa Litovchick |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Antigens
Polyomavirus Transforming Cell Cycle Proteins Simian virus 40 medicine.disease_cause Virus Replication Biochemistry 0302 clinical medicine Chlorocebus aethiops RNA Small Interfering Biology (General) 0303 health sciences Kv Channel-Interacting Proteins 3. Good health Host-Pathogen Interaction Lytic cycle 030220 oncology & carcinogenesis Receptors Virus RNA Interference Research Article QH301-705.5 Immunology Biology DNA-binding protein Microbiology Host Specificity Adenoviridae Cell Line 03 medical and health sciences Viral life cycle Virology Genetics medicine Animals Humans Protein Interactions Molecular Biology Gene 030304 developmental biology Gene Expression Profiling Proteins RC581-607 Molecular biology Protein Structure Tertiary Repressor Proteins Viral replication Virulence Factors and Mechanisms Trans-Activators Parasitology Heterologous expression Tumor Suppressor Protein p53 Immunologic diseases. Allergy |
Zdroj: | PLoS Pathogens, Vol 8, Iss 10, p e1002949 (2012) PLoS Pathogens |
ISSN: | 1553-7374 1553-7366 |
Popis: | The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains including the LXCXE or RB-binding motif, the DNA binding and helicase domains that contribute to the viral life cycle. In addition, the LT C-terminal region contains the host range and adenovirus helper functions required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells, identified interacting host proteins and carried out expression profiling. LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. Affinity purification of LT followed by mass spectrometry revealed a specific interaction between the LT C-terminal region and FAM111A, a previously uncharacterized protein. Depletion of FAM111A recapitulated the effects of heterologous expression of the LT C-terminal region, including increased viral gene expression and lytic infection of SV40 host range mutants and adenovirus replication in restrictive cells. FAM111A functions as a host range restriction factor that is specifically targeted by SV40 LT. Author Summary Viruses have evolved numerous mechanisms to counteract host cell defenses to facilitate productive infection. Simian Virus 40 (SV40) replication depends on specific interactions between large T antigen (LT) and a wide variety of host cell proteins. Although the LT C-terminal region has no evident enzymatic activity, mutations or deletions of this region significantly reduce the ability of the virus to replicate in restrictive cell types. Here, we identified host proteins that bind to LT and determined that the LT C-terminal region binds specifically to FAM111A. This physical interaction was required for efficient viral replication and sustained viral gene expression in restrictive cell types. In addition, RNAi-mediated knockdown of FAM111A levels in restrictive cells restored lytic infection of SV40 host range mutants and human adenovirus. These results indicate that FAM111A plays an important role in viral host range restriction. Our study provides insights into the viral-host perturbations caused by SV40 LT and the interaction of viruses with host restriction factors. |
Databáze: | OpenAIRE |
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