Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor

Autor: Megha Padi, Anna Korkhin, James A. DeCaprio, Robert L. James, Michael A. Calderwood, Rosa Yoon, Jarrod A. Marto, Marc Vidal, Luxuan Guo, Christian Berrios, Ying Zhang, Jingwei Cheng, Soundarapandian Velmurgan, Laurence Florens, Guillaume Adelmant, Michael E. Cusick, Debrah A. Fine, Michael P. Washburn, David E. Hill, Orit Rozenblatt-Rosen, Larisa Litovchick
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Antigens
Polyomavirus Transforming
Cell Cycle Proteins
Simian virus 40
medicine.disease_cause
Virus Replication
Biochemistry
0302 clinical medicine
Chlorocebus aethiops
RNA
Small Interfering
Biology (General)
0303 health sciences
Kv Channel-Interacting Proteins
3. Good health
Host-Pathogen Interaction
Lytic cycle
030220 oncology & carcinogenesis
Receptors
Virus
RNA Interference
Research Article
QH301-705.5
Immunology
Biology
DNA-binding protein
Microbiology
Host Specificity
Adenoviridae
Cell Line
03 medical and health sciences
Viral life cycle
Virology
Genetics
medicine
Animals
Humans
Protein Interactions
Molecular Biology
Gene
030304 developmental biology
Gene Expression Profiling
Proteins
RC581-607
Molecular biology
Protein Structure
Tertiary
Repressor Proteins
Viral replication
Virulence Factors and Mechanisms
Trans-Activators
Parasitology
Heterologous expression
Tumor Suppressor Protein p53
Immunologic diseases. Allergy
Zdroj: PLoS Pathogens, Vol 8, Iss 10, p e1002949 (2012)
PLoS Pathogens
ISSN: 1553-7374
1553-7366
Popis: The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains including the LXCXE or RB-binding motif, the DNA binding and helicase domains that contribute to the viral life cycle. In addition, the LT C-terminal region contains the host range and adenovirus helper functions required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells, identified interacting host proteins and carried out expression profiling. LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. Affinity purification of LT followed by mass spectrometry revealed a specific interaction between the LT C-terminal region and FAM111A, a previously uncharacterized protein. Depletion of FAM111A recapitulated the effects of heterologous expression of the LT C-terminal region, including increased viral gene expression and lytic infection of SV40 host range mutants and adenovirus replication in restrictive cells. FAM111A functions as a host range restriction factor that is specifically targeted by SV40 LT.
Author Summary Viruses have evolved numerous mechanisms to counteract host cell defenses to facilitate productive infection. Simian Virus 40 (SV40) replication depends on specific interactions between large T antigen (LT) and a wide variety of host cell proteins. Although the LT C-terminal region has no evident enzymatic activity, mutations or deletions of this region significantly reduce the ability of the virus to replicate in restrictive cell types. Here, we identified host proteins that bind to LT and determined that the LT C-terminal region binds specifically to FAM111A. This physical interaction was required for efficient viral replication and sustained viral gene expression in restrictive cell types. In addition, RNAi-mediated knockdown of FAM111A levels in restrictive cells restored lytic infection of SV40 host range mutants and human adenovirus. These results indicate that FAM111A plays an important role in viral host range restriction. Our study provides insights into the viral-host perturbations caused by SV40 LT and the interaction of viruses with host restriction factors.
Databáze: OpenAIRE
Externí odkaz: