Evidence for contribution of an internal ribosome entry site to intercellular transport of a tobamovirus
| Autor: | Pavel Ivanov, Maxim V. Skulachev, Joseph Atabekov, Yu. L. Dorokhov, A. G. Klyushin, S. D. Zvereva |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Base Sequence
biology Movement Intercellular transport Molecular Sequence Data Mutant Tobamovirus biology.organism_classification Ribosome Virology Molecular biology Plant Viral Movement Proteins Tobacco Mosaic Virus Viral Proteins Internal ribosome entry site Tobacco mosaic virus Movement protein Ribosomes Subgenomic mRNA |
| Zdroj: | Journal of General Virology. 85:1739-1744 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.79792-0 |
| Popis: | Previously, it has been shown that tobacco mosaic virus (TMV) U1 and crucifer-infecting TMV contain a 75 nt internal ribosome entry site (IRES) upstream of movement protein (MP) gene (IRES(U1)(MP,75) and IRES(CR)(MP,75), respectively). A movement-deficient TMV mutant, KK6, has been constructed previously [Lehto, K., Grantham, G. L.Dawson, W. O. (1990). Virology 174, 145-157] by insertion of the second coat protein subgenomic promoter (CP SGP-2) upstream of the MP gene, in addition to the natural CP SGP-1. Here, the authors compare the efficiency of movement function expression by KK6 and a derivative, K86, obtained by insertion of IRES(CR)(MP,75) between the CP SGP-2 and MP genes resulting in restoration of IRES(CR)(MP,75) function in the 5'-untranslated sequence of the I(2) subgenomic RNA of K86. The data indicate that the efficiency of K86 movement was largely restored by this insertion, which was apparently due to the translation-enhancing ability of IRES(CR)(MP,75). |
| Databáze: | OpenAIRE |
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