DEPs Induce Local Ige Class Switching Independent of Their Ability to Stimulate iBALT de Novo Formation
| Autor: | Dmitrii Borisovich Chudakov, Mariya Vladimirovna Konovalova, Elena Igorevna Kashirina, Olga Dmitrievna Kotsareva, Marina Alexandrovna Shevchenko, Daria Sergeevna Tsaregorodtseva, Gulnar Vaisovna Fattakhova |
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| Rok vydání: | 2022 |
| Předmět: |
diesel particulate matter
antibody production tertiary lymphoid structures local Ig class switch antigen doses lungs Mice Mice Inbred BALB C Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Hypersensitivity Animals Immunoglobulin E Immunoglobulin Class Switching Vehicle Emissions |
| Zdroj: | International Journal of Environmental Research and Public Health; Volume 19; Issue 20; Pages: 13063 |
| ISSN: | 1660-4601 |
| Popis: | Background: Diesel exhaust particles (DEPs) are leading to a general increase in atopic diseases worldwide. However, it is still unknown whether DEPs induce systemic B-cell IgE class switching in secondary lymphoid organs or locally in the lungs in inducible bronchus-associated lymphoid tissue (iBALT). The aim of this work was to identify the exact site of DEP-mediated B-cell IgE class switching and pro-allergic antibody production. Methods: We immunized BALB/c mice with different OVA doses (0.3 and 30 µg) intranasally in the presence and absence of two types of DEPs, SRM1650B and SRM2786. We used low (30 µg) and high (150 µg) DEP doses. Results: Only a high DEP dose induced IgE production, regardless of the particle type. Local IgE class switching was stimulated upon treatment with both types of particles with both low and high OVA doses. Despite the similar ability of the two standard DEPs to stimulate IgE production, their ability to induce iBALT formation and growth was markedly different upon co-administration with low OVA doses. Conclusions: DEP-induced local IgE class switching takes place in preexisting iBALTs independent of de novo iBALT formation, at least in the case of SRM1650B co-administered with low OVA doses. |
| Databáze: | OpenAIRE |
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