Heat shock protein 72 (Hsp72) specific induction and temporal stability in urine samples as a reliable biomarker of acute kidney injury (AKI)
| Autor: | Jonatan Barrera-Chimal, Luis E. Morales-Buenrostro, María Elena Flores, Rosalba Pérez-Villalva, Norma A. Bobadilla, Gerardo Gamba, Juan Antonio Ortega-Trejo, Diego Luis Carrillo-Pérez |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Kidney Cortex Health Toxicology and Mutagenesis Renal cortex Urinary system Blotting Western Clinical Biochemistry Urology Enzyme-Linked Immunosorbent Assay HSP72 Heat-Shock Proteins Urine Kidney urologic and male genital diseases Sensitivity and Specificity Biochemistry Ischemia medicine Animals Humans Rats Wistar Renal ischemia Protein Stability business.industry Temperature Acute kidney injury Acute Kidney Injury medicine.disease Surgery medicine.anatomical_structure Reperfusion Injury Biomarker (medicine) business Reperfusion injury Biomarkers |
| Zdroj: | Biomarkers. 20:453-459 |
| ISSN: | 1366-5804 1354-750X |
| DOI: | 10.3109/1354750x.2015.1096305 |
| Popis: | We demonstrated that urinary heat shock protein of 72 KDa (Hsp72) is a sensitive biomarker for the early detection of acute kidney injury (AKI). However, whether Hsp72 induction during an AKI episode is kidney-specific is unknown, as well as, the degree of Hsp72 stability in urine samples. In rats that underwent bilateral renal ischemia and reperfusion (I/R), Hsp72 levels were evaluated in several tissues and in collected urines under different storage and temperature conditions, as well as in variable numbers of freeze-thaw cycles. The effect of room temperature and five freeze-thaw cycles on urinary Hsp72 levels was also evaluated in urine samples from AKI patients. We found that Hsp72 increased exclusively in the renal cortex of I/R group, emphasizing its performance as an AKI biomarker. Urinary-Hsp72 remained constant at room temperature (48 h), during 9 months of storage and was not affected by five freeze/thaw cycles. |
| Databáze: | OpenAIRE |
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