Cloning and Nucleotide Sequence of Amidase Gene fromPseudomonas putida
| Autor: | Robert D. Fallon, Shijun Wu, Mark S. Payne |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Operon
Molecular Sequence Data Restriction Mapping Regulatory Sequences Nucleic Acid Biology medicine.disease_cause Amidohydrolases Amidase Nitrile hydratase Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Molecular Biology Gene Base Sequence Pseudomonas putida Nucleic acid sequence Cell Biology General Medicine biology.organism_classification Recombinant Proteins Molecular Weight Open reading frame Biochemistry |
| Zdroj: | DNA and Cell Biology. 17:915-920 |
| ISSN: | 1557-7430 1044-5498 |
| Popis: | Amidases are a class of enzymes which convert amides to acids and have potential value in the development of commercial bioprocesses for the production of useful chemicals. A gene encoding an amidase in Pseudomonas putida 5B has been cloned, sequenced, and overexpressed in Escherichia coli. An additional open reading frame (P38K) encoding a putative protein of 38 kDa was found immediately upstream of the amidase gene. This work continues our characterization of a P. putida operon, which now appears to include P38K, amidase, and a stereo-specific nitrile hydratase. This characterization underlies continuing efforts in biocatalyst development. |
| Databáze: | OpenAIRE |
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