ATP-induced, sustained calcium signalling in cultured rat cortical astrocytes: evidence for a non-capacitative, P2X7-like-mediated calcium entry
| Autor: | Carlo Cugnoli, Susanna Alloisio, Ilaria Monaldi, Mario Nobile, Stefano Ferroni |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Purinergic P2 Receptor Agonists
Agonist medicine.drug_class Suramin Biophysics Biochemistry Astroglia Microfluorimetry chemistry.chemical_compound Adenosine Triphosphate Structural Biology Calcium influx Purinergic P2 Receptor Antagonists Genetics medicine Animals Calcium Signaling P2 purinoceptor Receptor Molecular Biology Cells Cultured Calcium signaling Cerebral Cortex Receptors Purinergic P2 Chemistry Cell Biology Rats Cytosol medicine.anatomical_structure Astrocytes Pyridoxal Phosphate P2X7 receptor Receptors Purinergic P2X7 Adenosine triphosphate medicine.drug Astrocyte |
| Zdroj: | FEBS Letters. 538:71-76 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(03)00129-7 |
| Popis: | The receptor mechanisms regulating the ATP-induced free cytosolic Ca(2+) concentration ([Ca(2+)](i)) changes in cultured rat cortical type-1 astrocytes were analyzed using fura-2-based Ca(2+) imaging microscopy. Upon prolonged ATP challenge (1-100 microM), astroglial cells displayed a biphasic [Ca(2+)](i) response consisting of an initial peak followed by a sustained elevation. Suramin and pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid blocked both components, albeit to a different extent. By contrast, the selective P2X7 antagonist oxidized ATP irreversibly abrogated the sustained [Ca(2+)](i) signal without affecting the transient phase. Finally, astrocyte challenge with the selective P2X7 agonist 3'-O-(4-benzoyl)benzoyl-ATP evoked a sustained [Ca(2+)](i) elevation, which occluded that induced by ATP. We can conclude that in cultured cortical astrocytes the ATP-mediated sustained [Ca(2+)](i) rise does not implicate capacitative Ca(2+) entry but involves Ca(2+) influx through P2X7-like receptors. |
| Databáze: | OpenAIRE |
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