MicroRNA-195-5p is a potential diagnostic and therapeutic target for breast cancer
| Autor: | Chuankui Wei, Yixiang Huang, Qifeng Luo, Lei Chen, Lin Fang, Dengfeng Li, Xiaoyu Li, Jia Li, Hongming Song |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Gene Expression Breast Neoplasms Biology medicine.disease_cause breast cancer Breast cancer Cell Movement Cell Line Tumor Cyclin E microRNA Biomarkers Tumor medicine Humans Cell Proliferation miR-195-5p Oncogene Proteins Oncogene Carcinoma Ductal Breast Cancer Articles General Medicine Cell cycle medicine.disease G1 Phase Cell Cycle Checkpoints Molecular biology Gene Expression Regulation Neoplastic MicroRNAs HEK293 Cells Real-time polymerase chain reaction Oncology Cancer cell Female RNA Interference Carcinogenesis |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2014.2971 |
| Popis: | MicroRNAs (miRNAs) are a class of highly conserved, small endogenous single-strand non-coding RNAs. They are aberrantly expressed in the circulation and tissue of patients with cancer. Therefore, it has been suggested that they may act as key regulators of carcinogenesis. The aim of the present study was to examine the expression level of miR-195-5p in human breast cancer and its potential role in carcinogenesis. The expression level of miR-195-5p was measured in 40 breast cancer specimens and adjacent normal breast tissues by quantitative polymerase chain reaction (qPCR). Next, to explore the potential function of miR-195-5p, we used MDA-MB-231 human breast cancer cells and carried out MTT, colony formation, Transwell chamber migration and cell cycle assays. The dual-luciferase reporter assay was also performed to determine putative targets of miR-195-5p, which were validated using qPCR and western blot assays. We found that miR-195-5p expression was significantly decreased in the 40 breast cancer specimens when compared with that in the adjacent normal breast tissues (P |
| Databáze: | OpenAIRE |
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