Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms
| Autor: | Daniella Alchaar D’Ávila, Eliane Lages-Silva, Lúcia Maria da Cunha Galvão, Egler Chiari, Wendell Sérgio Ferreira Meira, Eliane Dias Gontijo |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Chagas disease DNA polymerase Trypanosoma cruzi Polymorphism (computer science) parasitic diseases medicine Animals Cluster Analysis Humans Chagas Disease Genetic variability Child Phylogeny Genetics Polymorphism Genetic General Veterinary biology Kinetoplastida General Medicine DNA Protozoan Middle Aged medicine.disease biology.organism_classification DNA Fingerprinting Random Amplified Polymorphic DNA Technique RAPD Infectious Diseases DNA profiling Insect Science biology.protein Female Parasitology Brazil |
| Zdroj: | Parasitology Research. 98:455-461 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-005-0043-5 |
| Popis: | The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically. |
| Databáze: | OpenAIRE |
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