Novel DNA structures resulting from dTam3 excision in tobacco
| Autor: | Steve Scofield, H. John J. Nijkamp, Jacques Hille, Marianne J. Teeuwen-de Vroomen, Gerjan S. Leuring, Michel A. Haring |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, SILS (FNWI) |
| Rok vydání: | 1991 |
| Předmět: |
Transcriptional Activation
Tam3 Molecular Sequence Data Restriction Mapping Plant Science Biology medicine.disease_cause Genome Transposition (music) chemistry.chemical_compound Restriction map Tobacco Escherichia coli transposition Genetics medicine rearrangements two-element system Cloning Molecular Gene Gene Rearrangement Base Composition Base Sequence Phenotypic assay General Medicine Gene rearrangement Plants Genetically Modified Molecular biology Mutagenesis Insertional Plants Toxic Oligodeoxyribonucleotides chemistry DNA Transposable Elements excision sites Agronomy and Crop Science DNA Rhizobium |
| Zdroj: | Plant Molecular Biology, 17(5) Plant Molecular Biology, 17, 995-1004. Springer Netherlands |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00037139 |
| Popis: | A Tam3 two-element system has been designed by combining an immobilized Tam3 element with a non-autonomous dTam3 element inserted into the HPT gene. The phenotypic assay employed, restored hygromycin resistance, indicated that trans-activation of the non-autonomous dTam3 element occurred. Molecular analyses of the excision sites revealed that the ends of the dTam3 element remain in the empty donor sites. The predominant consequence of this type of excision appears to be that excised fragments fail to re-integrate into the tobacco genome. Only one case of dTam3 re-integration could be detected. The ends of this element had been degraded upon integration into the tobacco genome. Either the altered structure of the Tam3 derivatives or tobacco host factors are influencing the trans-activation of a dTam3 element, resulting in aberrant excision. |
| Databáze: | OpenAIRE |
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