Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes
| Autor: | Danica Petrovic-Djergovic, Tad Eichler, Seetharamaiah Chittiprol, Richard F. Ransom, Phylip Chen |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Physiology Cellular differentiation Motility Puromycin Aminonucleoside Biology Cell Line Podocyte Rats Sprague-Dawley Nephrin Mice Species Specificity Cell Movement medicine Animals Humans WT1 Proteins Cells Cultured Cell Proliferation Cell Death Podocytes Microfilament Proteins Intracellular Signaling Peptides and Proteins Membrane Proteins Cell Differentiation Articles Molecular biology Rats Cell biology medicine.anatomical_structure Membrane protein Doxorubicin Cell culture Models Animal Podocin biology.protein Synaptopodin Biomarkers |
| Zdroj: | American Journal of Physiology-Renal Physiology. 301:F660-F671 |
| ISSN: | 1522-1466 1931-857X |
| Popis: | The state-of-the-art cultured podocyte is conditionally immortalized by expression of a temperature-sensitive mutant of the SV40 large-T antigen. These cultures proliferate at 33°C and differentiate at 37°C into arborized cells that more closely resemble in vivo podocytes. However, the degree of resemblance remains controversial. In this study, several parameters were measured in podocyte cell lines derived from mouse (JR, KE), human (MS), and rat (HK). In all lines, the quantities of NEPH1 and podocin proteins and NEPH1 and SYNPO mRNAs were comparable to glomeruli, while synaptopodin and nephrin proteins and NPHS1 and NPHS2 mRNAs were > KE, and of doxorubicin was JR ∼ KE > MS, while HK cells were almost unaffected. Process formation was largely a result of contractile action after formation of lamellipodia. These findings demonstrate dramatic differences in marker expression, response to toxins, and motility between lines of podocytes from different species and even between similarly-derived mouse lines. |
| Databáze: | OpenAIRE |
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