Differential Use of Human Neutrophil Fcγ Receptors for Inducing Neutrophil Extracellular Trap Formation
| Autor: | Nancy Mora, Ricarda Cortes-Vieyra, Eileen Uribe-Querol, Carlos Rosales, Omar Rafael Alemán |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy Extracellular Traps Integrins Article Subject MAP Kinase Signaling System Neutrophils Immunology Integrin GPI-Linked Proteins 03 medical and health sciences chemistry.chemical_compound Phagocytosis Immunology and Allergy Humans Receptor Protein kinase C biology Receptors IgG Degranulation General Medicine Neutrophil extracellular traps Chromatin Cell biology 030104 developmental biology chemistry Phorbol biology.protein Tetradecanoylphorbol Acetate Signal transduction Reactive Oxygen Species lcsh:RC581-607 Research Article Signal Transduction |
| Zdroj: | Journal of Immunology Research, Vol 2016 (2016) Journal of Immunology Research |
| ISSN: | 2314-7156 2314-8861 |
| Popis: | Neutrophils (PMN) are the most abundant leukocytes in the blood. PMN migrate from the circulation to sites of infection, where they are responsible for antimicrobial functions. PMN use phagocytosis, degranulation, and formation of neutrophil extracellular traps (NETs) to kill microbes. NETs are fibers composed of chromatin and neutrophil-granule proteins. Several pathogens, including bacteria, fungi, and parasites, and also some pharmacological stimuli such as phorbol 12-myristate 13-acetate (PMA) are efficient inducers of NETs. Antigen-antibody complexes are also capable of inducing NET formation. However the particular Fcγreceptor involved in triggering this function is a matter of controversy. In order to provide some insight into what Fcγreceptor is responsible for NET formation, each of the two human Fcγreceptors was stimulated individually by specific monoclonal antibodies and NET formation was evaluated. FcγRIIa cross-linking did not promote NET formation. Cross-linking other receptors such as integrins also did not promote NET formation. In contrast FcγRIIIb cross-linking induced NET formation similarly to PMA stimulation. NET formation was dependent on NADPH-oxidase, PKC, and ERK activation. These data show that cross-linking FcγRIIIb is responsible for NET formation by the human neutrophil. |
| Databáze: | OpenAIRE |
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