A critical evaluation of the contamination, by lysosomes, of preparations of outer membrane of mitochondria
| Autor: | P.M. Vignais, J. Nachbaur |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Acid Phosphatase
Biophysics Centrifugation Mitochondria Liver Mitochondrion Biochemistry Electron Transport Complex IV Surface-Active Agents Mitochondrial membrane transport protein Lysosome Centrifugation Density Gradient Methods medicine Animals Inner mitochondrial membrane Monoamine Oxidase Molecular Biology Differential centrifugation Phospholipase A Membranes biology Cell Biology Rats medicine.anatomical_structure Membrane Liver Glucose-6-Phosphatase biology.protein Sulfatases Lysosomes Bacterial outer membrane |
| Zdroj: | Biochemical and Biophysical Research Communications. 33:307-314 |
| ISSN: | 0006-291X |
| Popis: | Several methods have been recently set up (cf. Quagliariello et al. , 1967 ) to separate inner and outer membranes of mitochondria by density gradient centrifugation. Although these methods are powerful tools for the study of mitochondrial compartmentation, their validity must still be critically examined on morphological and enzymatic criteria. In this line of idea, Parsons et al. (1967) have evaluated the degree of microsomal contamination of mitochondrial membrane fractions. Other membranes such as lysosomal membranes may also be contaminant. In the course of a study undertaken in this laboratory to specify the localization and to understand the role of phospholipase A in mitochondrial membranes it became necessary to assess the purity of mitochondrial membrane preparations and especially to avoid as much as possible a contamination by lysosomes. We thought it useful to present here some observations we made when using enzymic markers to evaluate the degree of contamination of membrane preparations which would appear homogeneous under the electron microscope. The results presented in this paper show that on discontinuous sucrose gradient lysosome membranes gather at the same place as outer mitochondrial membranes. Based on a previous work by Wattiaux, Wibo and Baudhuin (1963) , a procedure is described which leads to a preparation of outer membranes of mitochondria practically devoid of lysosomes. |
| Databáze: | OpenAIRE |
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